ZFIN ID: ZDB-FIG-170809-7
Mesureur et al., 2017 - Macrophages, but not neutrophils, are critical for proliferation of Burkholderia cenocepacia and ensuing host-damaging inflammation. PLoS pathogens   13:e1006437 Full text @ PLoS Pathog.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Protruding-mouth

Fig. 6

Macrophages, but not neutrophils, contribute to increased bacterial burden and pro-inflammatory responses towards subcutaneously introduced B. cenocepacia.

(A) Mpeg1/umn+ embryos were subcutaneously injected with B. cenocepacia K56-2 expressing Turquoise. Fluorescent overlay images were taken at 90 min and 5 h post infection, showing infected macrophages (red). Scale bars, 10 μm. (B) Images (red and blue overlay and below slightly enlarged individual fluorescence images with blue filter) of the indicated area (see drawing) of an mpx/umn+ (B1) and an mpeg1/umn+ (B2) embryo subcutaneously injected with B. cenocepacia K56-2 expressing Turquoise. B1 shows an embryo followed in time displaying neutrophil infiltration (red) and increase in bacterial burden (blue, see Fig 7A for quantification). B2 shows the image of an embryo with macrophage infiltration (red) and high bacterial burden at 24 hpi. Arrow head points at mCherry positive debris. See non-infected mpx/umn+ and mpeg1/umn+ control embryos in S6 Fig for comparison. Scale bars, 100 μm. (C) Image of the infected area of a representative mpx/umn+ embryo depleted of neutrophils with Mtz, and subcutaneously injected with B. cenocepacia K56-2 (Turquoise) at 24 hpi. Scale bar 100 μm, and 50 μm for inset. See also S6D Fig. (D) Image of a representative mpeg1/umn+ embryo depleted of macrophages with Mtz and subcutaneously injected with B. cenocepacia K56-2 (Turquoise) at 24 hpi. Scale bar 100 μm, and 50 μm for inset.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mCherry gl24Tg; i149Tg; i186Tg; s1999tTg (AB) bacterial treatment by injection: Burkholderia cenocepacia Long-pec macrophage IFL
Protruding-mouth macrophage IFL
gl24Tg; i149Tg; i186Tg; s1999tTg (AB) bacterial treatment by injection: Burkholderia cenocepacia, chemical ablation: macrophage, chemical treatment by environment: metronidazole Protruding-mouth Not Detected macrophage IFL
i149Tg; i186Tg; s1999tTg; sh267Tg (AB) bacterial treatment by injection: Burkholderia cenocepacia Long-pec to Protruding-mouth neutrophil IFL
i149Tg; i186Tg; s1999tTg; sh267Tg (AB) chemical ablation: neutrophil, bacterial treatment by injection: Burkholderia cenocepacia, chemical treatment by environment: metronidazole Protruding-mouth Not Detected neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gl24Tg; i149Tg; i186Tg; s1999tTg (AB) bacterial treatment by injection: Burkholderia cenocepacia, chemical ablation: macrophage, chemical treatment by environment: metronidazole Protruding-mouth macrophage decreased amount, abnormal
Protruding-mouth macrophage mCherry expression absent, abnormal
i149Tg; i186Tg; s1999tTg; sh267Tg (AB) chemical ablation: neutrophil, bacterial treatment by injection: Burkholderia cenocepacia, chemical treatment by environment: metronidazole Protruding-mouth neutrophil decreased amount, abnormal
Protruding-mouth neutrophil mCherry expression absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.