FIGURE

Fig. 1

ID

ZDB-FIG-170530-4

Publication

Chow et al., 2017 - In Vivo Measurement of Glycine Receptor Turnover and Synaptic Size Reveals Differences between Functional Classes of Motoneurons in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

The Expression of dendra2-GlyRα1 in Motoneurons, Physiology of dendra2-GlyRα1 Channels in Muscle Fibers, and Synapse Level Targeting for dendra2 Photoconversion

(A) A single motoneuron expressing dendra2-GlyRα1 (green) along with membrane targeted tdTomato (magenta), exhibiting distributed glycinergic puncta along the labeled soma and dendrites. See also Figure S1 for costaining with gephyrin.

(B) Expression of dendra2-GlyRα1 in muscle to test channel formation. Left: transgenic muscle fiber targeted for patch recording. Right: voltage-clamp recordings at the indicated holding potentials in a muscle fiber.

(C) Precise targeting with a 405-nm laser allowed us to convert synaptically localized dendra2-GlyRα1 from green (top) to red fluorescence (bottom, represented as magenta) with near single-synapse accuracy.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.