ZFIN Image: Chow et al., 2017, Fig. 1

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Figure Caption

Fig. 1

The Expression of dendra2-GlyRα1 in Motoneurons, Physiology of dendra2-GlyRα1 Channels in Muscle Fibers, and Synapse Level Targeting for dendra2 Photoconversion

(A) A single motoneuron expressing dendra2-GlyRα1 (green) along with membrane targeted tdTomato (magenta), exhibiting distributed glycinergic puncta along the labeled soma and dendrites. See also Figure S1 for costaining with gephyrin.

(B) Expression of dendra2-GlyRα1 in muscle to test channel formation. Left: transgenic muscle fiber targeted for patch recording. Right: voltage-clamp recordings at the indicated holding potentials in a muscle fiber.

(C) Precise targeting with a 405-nm laser allowed us to convert synaptically localized dendra2-GlyRα1 from green (top) to red fluorescence (bottom, represented as magenta) with near single-synapse accuracy.

Acknowledgments

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