ZFIN ID: ZDB-FIG-170424-19
Yang et al., 2017 - Miconazole protects blood vessels from matrix metalloproteinase 9-dependent rupture and hemorrhage. Disease models & mechanisms   10(3):337-348 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 1

The fn40a mutant is a hemorrhagic stroke model suitable for chemical suppressor screens in zebrafish. (See also Figs S1,S2). (A-D) Live images of wild-type siblings (A,B) and homozygous fn40a mutants (C,D) at 2 dpf; note evident brain hemorrhages in the mutant (arrows). A,C: lateral view; B,D: dorsal view. (E-L) Live images of Tg(flk1:eGFP); Tg(gata1:DsRed) double transgenic embryos of wild-type siblings (E,F,I,J) and fn40a mutants (G,H,K,L) at 2 dpf. Note that gata1+ erythrocytes leak from Tg(flk1:eGFP)-labeled vessels and form hematomas in the mutants (G-H,K; arrow) while erythrocytes remain within vessels in wild-type siblings (E,F,I). Tg(coro1a:eGFP)-labeled macrophages and neutrophils accumulate at the hemorrhagic site in this mutant (L, arrow) whereas leukocytes are evenly distributed in the brain of a wild-type sibling embryo (J) at 2 dpf (n>8). (M) Overview of the chemical screen with mutant embryos arrayed in a 96-well plate and treated with DMSO or compound, illustrating that miconazole suppresses brain hemorrhages in a dose-dependent manner compared with DMSO treatment. Red color indicates hemorrhagic mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed arhgef7bf40a/f40a; s843Tg; sd2Tg standard conditions Long-pec brain nucleate erythrocyte IFL
s843Tg; sd2Tg standard conditions Long-pec brain nucleate erythrocyte IFL
EGFP arhgef7bf40a/f40a; hkz04tTg standard conditions Long-pec brain macrophage IFL
Long-pec brain neutrophil IFL
arhgef7bf40a/f40a; s843Tg; sd2Tg standard conditions Long-pec brain vasculature IFL
hkz04tTg standard conditions Long-pec brain macrophage IFL
Long-pec brain neutrophil IFL
s843Tg; sd2Tg standard conditions Long-pec brain vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
arhgef7bf40a/f40a standard conditions Long-pec brain hemorrhagic, abnormal
arhgef7bf40a/f40a; hkz04tTg standard conditions Long-pec leukocyte accumulation brain, abnormal
arhgef7bf40a/f40a; s843Tg; sd2Tg standard conditions Long-pec brain hemorrhagic, abnormal
Long-pec central artery structure, abnormal
Long-pec nucleate erythrocyte accumulation brain, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.