IMAGE

Fig. 1

ID
ZDB-IMAGE-170424-18
Genes
Source
Figures for Yang et al., 2017
Image
Figure Caption

Fig. 1

The fn40a mutant is a hemorrhagic stroke model suitable for chemical suppressor screens in zebrafish. (See also Figs S1,S2). (A-D) Live images of wild-type siblings (A,B) and homozygous fn40a mutants (C,D) at 2 dpf; note evident brain hemorrhages in the mutant (arrows). A,C: lateral view; B,D: dorsal view. (E-L) Live images of Tg(flk1:eGFP); Tg(gata1:DsRed) double transgenic embryos of wild-type siblings (E,F,I,J) and fn40a mutants (G,H,K,L) at 2 dpf. Note that gata1+ erythrocytes leak from Tg(flk1:eGFP)-labeled vessels and form hematomas in the mutants (G-H,K; arrow) while erythrocytes remain within vessels in wild-type siblings (E,F,I). Tg(coro1a:eGFP)-labeled macrophages and neutrophils accumulate at the hemorrhagic site in this mutant (L, arrow) whereas leukocytes are evenly distributed in the brain of a wild-type sibling embryo (J) at 2 dpf (n>8). (M) Overview of the chemical screen with mutant embryos arrayed in a 96-well plate and treated with DMSO or compound, illustrating that miconazole suppresses brain hemorrhages in a dose-dependent manner compared with DMSO treatment. Red color indicates hemorrhagic mutants.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.