FIGURE

Fig. 1

ID

ZDB-FIG-170420-8

Publication

Zhu et al., 2017 - Vegfa Impacts Early Myocardium Development in Zebrafish

Other Figures

(all 8)

All Figure Page

Back to All Figure Page

Fig. 1

Vascular endothelial growth factor Aa (vegfaa) deficiency disrupts myocardial and endocardial morphogenesis. (A–I) Fluorescence micrographs of the hearts of wild-type (A–C), vegfaa mutant (D–F), and vegfaa morphants (G–I) at 96 hpf (hours post-fertilization) with the background of Tg(myl7:mCherry)/Tg(kdrl:EGFP) transgenic fish. The whole heart is substantially small and linearized in the mutant and the morphant, and no cavity is visible inside the heart lumen. Scale bar: 50 μm (A– I); (J,K) In situ hybridization of vegfaa in wild-type embryos at 18-somite stage. vegfaa is expressed in anterior lateral plate mesoderm as indicated by the arrow. (J) Lateral views, (K) Dorsal views with the anterior at the top. Scale bar: 200 μm (J,K). WT, wild-type; mo, morpholino

Expression Data

Genes:	GFP mCherry vegfaa
Fish:	AB la116Tg; sd7Tg la116Tg; sd7Tg + MO9-vegfaa vegfaa^{zf1090/zf1090}; la116Tg; sd7Tg
Knockdown Reagent:	MO9-vegfaa
Anatomical Terms:	anterior lateral plate mesoderm endocardium myocardium
Stage Range:	14-19 somites to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg; sd7Tg + MO9-vegfaa vegfaa^{zf1090/zf1090}; la116Tg; sd7Tg
Knockdown Reagent:	MO9-vegfaa
Observed In:	atrium cardiac ventricle endocardium endothelial cell heart heart anatomical space heart tube formation heart looping heart valve (all 8)
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.