FIGURE

Fig. 7

ID
ZDB-FIG-170315-25
Publication
Ye et al., 2015 - Endoderm convergence controls subduction of the myocardial precursors during heart-tube formation
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

Epithelial organization of myocardial cells is disrupted in Gα13 morphants. (A-E) Whole-mount ZO-1 immunostaining was performed in embryos indicated at 12 s and 16 s. (A-D) Projections of confocal z-stacks of the right lateral mesoderm showing ZO-1 expression (gray) in the nkx2.5:Kaede-expressing (myocardial) cells (green). (A′-D′) Higher-magnification views of areas shown in boxes in A-D. The vertices were defined as regions where membranes from three adjacent cells come into contact, and the edges as the cell periphery excluding the vertices. Yellow dashed line, midline; circles, vertices; arrowheads, edges. (E) Intensity of ZO-1 expression in myocardial cells at the vertices and edges in control embryos (54 cells from 7 embryos at 12 s and 25 cells from 4 embryos at 16 s) and gna13a/b MO-injected embryos (51 cells from 8 embryos at 12 s and 20 cells from 4 embryos). *P<0.001 versus control. Data are mean±s.e.m. (F-K) Transverse vibratome sections immunostained for Kaede (magenta), ZO-1 (green), actin (Rhodamine-Phalloidin, red) and nuclei (DAPI, blue). Yellow dashed line indicates midline; white dashed lines outline myocardial cells; white and yellow arrowheads indicate normal and ectopic ZO-1 expression, respectively. Scale bars: 20 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development