ZFIN ID: ZDB-FIG-170209-31
Barske et al., 2016 - Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face. PLoS Genetics   12:e1005967 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Day 5

Fig. 9

Simultaneous loss of prrx1a/b and jag1b further improves ventral cartilages in edn1 mutants.

(A-E) Ventral views of dissected facial skeletons. edn1 mutants have much reduced ventral cartilage in the first (black closed arrowhead) and second (black open arrowhead) arches. Whereas jag1b; edn1 and prrx1a; prrx1b; edn1 mutants show some restoration of ventral cartilage, primarily in the second arch, quadruple prrx1a; prrx1b; jag1b; edn1 mutants show a prominent rescue of second arch Ch cartilage (black open arrowhead) and increased length of first arch M cartilage (black closed arrowhead). (F) Quantification of skeletal rescue in prrx1a; prrx1b; edn1 mutants (left column) and jag1b; edn1 mutants with decreasing numbers of wild-type prrx1a/b alleles (e.g. 4 wild-type alleles = pure jag1b; edn1 mutants; 0 wild-type alleles = quadruple mutant). Examples of no, mild, moderate, or strong rescue of the ceratohyal cartilage are shown in (B-E). Numbers in each genotype, from left to right: 11, 13, 17, 15, 6. (G-J) edn1 mutants show a loss of ventral barx1 expression (magenta, white open arrowhead) and a partial reduction of dlx5a (green), which are partially restored in prrx1a; prrx1b; edn1 triple mutants. The magenta channel is shown by itself in (G'-J'). Scale bar in E = 100 μm; scale bar in J' = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barx1 WT control Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region ISH
edn1tf216b/tf216b; prrx1ael558/el558; el49Tg/el49Tg standard conditions Prim-25 pharyngeal arch ventral region ISH
prrx1ael558/el558; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch ventral region ISH
dlx5a WT control Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region ISH
edn1tf216b/tf216b; prrx1ael558/el558; el49Tg/el49Tg standard conditions Prim-25 pharyngeal arch ventral region ISH
prrx1ael558/el558; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ventral region barx1 expression decreased amount, abnormal
Prim-25 pharyngeal arch ventral region dlx5a expression decreased amount, abnormal
Day 5 pharyngeal arch 1 ventral region decreased amount, abnormal
Day 5 pharyngeal arch 1 ventral region decreased size, abnormal
Day 5 pharyngeal arch 2 ventral region decreased amount, abnormal
Day 5 pharyngeal arch 2 ventral region decreased size, abnormal
Day 5 pharyngeal arch cartilage ventral region decreased amount, abnormal
Day 5 pharyngeal arch cartilage ventral region morphology, abnormal
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Day 5 pharyngeal arch 1 ventral region morphology, abnormal
Day 5 pharyngeal arch 2 ventral region morphology, abnormal
Day 5 pharyngeal arch 2 ventral region size, ameliorated
Day 5 pharyngeal arch cartilage ventral region amount, ameliorated
Day 5 pharyngeal arch cartilage ventral region morphology, abnormal
edn1tf216b/tf216b; jag1bb1105/b1105; prrx1ael558/el558; prrx1bel491/el491 standard conditions Day 5 ceratohyal cartilage size, ameliorated
Day 5 Meckel's cartilage length, ameliorated
Day 5 pharyngeal arch cartilage ventral region morphology, abnormal
edn1tf216b/tf216b; prrx1ael558/el558; el49Tg/el49Tg standard conditions Prim-25 pharyngeal arch ventral region barx1 expression amount, ameliorated
Prim-25 pharyngeal arch ventral region dlx5a expression amount, ameliorated
Day 5 pharyngeal arch 1 ventral region morphology, abnormal
Day 5 pharyngeal arch 2 ventral region morphology, abnormal
Day 5 pharyngeal arch 2 ventral region size, ameliorated
Day 5 pharyngeal arch cartilage ventral region amount, ameliorated
Day 5 pharyngeal arch cartilage ventral region morphology, abnormal
prrx1ael558/el558; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch ventral region barx1 expression decreased amount, abnormal
Prim-25 pharyngeal arch ventral region dlx5a expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.