ZFIN ID: ZDB-FIG-170209-29
Barske et al., 2016 - Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face. PLoS Genetics   12:e1005967 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Day 5

Fig. 7

Combined loss of prrx1a and prrx1b results in ectopic dorsal cartilage.

(A-F) prrx1a; prrx1b mutants develop ectopic cartilage, both from the dorsal-medial surface of Pq (black arrow) and connecting Pq to the otic cartilage (magenta arrowheads), as well as fusions of Hm to the otic cartilage (yellow arrows). The entopterygoid dermal bone (black arrowhead) that normally forms along the dorsal-medial surface of the Pq is also lost. (G, H) In prrx1a; prrx1b mutants at 36 hpf, barx1 (magenta) is ectopically upregulated in the dorsal first arch (white arrowhead), along the dorsal border of the second arch (white bracket, compare with G), and in the posterior dorsal second arch (white arrow). (I, J) By 48 hpf in prrx1a; prrx1b mutants, ectopic barx1 expression is no longer evident in the posterior first arch (open white arrowhead), and the second arch barx1+ domain is slightly larger than the sibling control (white arrow). sox9a expression (green) is largely normal at this stage in prrx1a; prrx1b mutants. Scale bars in B and F = 100 μm; scale bars in H and J = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barx1 WT control Prim-25 pharyngeal arch ISH
Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
Long-pec pharyngeal arch ISH
prrx1ael558/el558; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal margin ISH
Prim-25 pharyngeal arch 2 postero-dorsal region ISH
Long-pec pharyngeal arch 1 ISH
Long-pec pharyngeal arch 2 ISH
sox9a WT control Long-pec pharyngeal arch ISH
prrx1ael558/el558; prrx1bel491/el491 standard conditions Long-pec pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
prrx1ael558/el558; prrx1bel491/el491 standard conditions Prim-25 pharyngeal arch 1 dorsal region barx1 expression increased amount, abnormal
Prim-25 pharyngeal arch 1 dorsal region barx1 expression increased distribution, abnormal
Prim-25 pharyngeal arch 1 dorsal region barx1 expression mislocalised, abnormal
Prim-25 pharyngeal arch 2 dorsal margin barx1 expression increased amount, abnormal
Prim-25 pharyngeal arch 2 dorsal margin barx1 expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 dorsal margin barx1 expression mislocalised, abnormal
Prim-25 pharyngeal arch 2 postero-dorsal region barx1 expression increased amount, abnormal
Prim-25 pharyngeal arch 2 postero-dorsal region barx1 expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 postero-dorsal region barx1 expression mislocalised, abnormal
Long-pec pharyngeal arch 2 barx1 expression increased distribution, abnormal
Day 5 entopterygoid absent, abnormal
Day 5 hyomandibula fused with inner ear, abnormal
Day 5 hyomandibula morphology, abnormal
Day 5 palatoquadrate cartilage fused with inner ear, abnormal
Day 5 palatoquadrate cartilage mislocalised, abnormal
Day 5 palatoquadrate cartilage morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.