Fig. 4

Decreased hair cell regeneration in JNK inhibition zebrafish can be partly rescued by over-activating Wnt signalling. (A–B) The FM1-43FX labelled hair cells are significantly increased in apc mutant zebrafish compared to control larvae in SP600125-treated and untreated larvae at 24 h after neomycin treatment. Scale bar = 10 μm. (C) Quantification of FM1-43FX-positive hair cells. *p < 0.05; **p < 0.001. (n = 53 neuromasts from the non-neomycin-treated 5 dpf control animals, n = 44 neuromasts from the non-neomycin-treated apc mutant zebrafish at 5 dpf, n = 72 neuromasts from the control larvae at 24 h following neomycin damage, n = 11 neuromasts from apc mutant larvae at 24 h following neomycin damage, n = 21 neuromasts from 15 μM SP600125-treated larvae at 24 h following neomycin damage, n = 63 neuromasts from 15 μM SP600125-treated apc mutant larvae at 24 h following neomycin damage. Con 5 dpf vs. apc mutant 5 dpf: unpaired t test, two-tailed, t = 11.39, df = 95, p < 0.001; Con neo-24 h vs. apc neo-24 h: unpaired t test, two-tailed, t = 2.767, df = 81, p = 0.007; Con neo SP600125-24 h vs. apc neo SP600125-24 h: unpaired t test, two-tailed, t = 5.186, df = 82, p < 0.0001).