ZFIN ID: ZDB-FIG-170111-8
Begay et al., 2016 - FLNC Gene Splice Mutations Cause Dilated Cardiomyopathy.. JACC. Basic to translational science   1:344-359 Full text @ JACC Basic Transl Sci
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Days 7-13

Fig. 4

MO Knockdown of flncb in Zebrafish Showing a Cardiac Phenotype

(A) Black bars: percentage of surviving embryos with severe cardiac phenotype at 72 h post-fertilization (hpf) for uninjected (n = 411), p53 morpholino (MO)-injected (n = 139), 25-N+p53 control MO-injected (n = 351), and filamin Cb (flncb)+p53 MO-injected (n = 241); *p = 1.04 × 10-83 (for comparison of uninjected heart vs. flncb+p53 MO using chi-square testing). Gray bars: percentage of surviving embryos at 7 days for uninjected (n = 411), p53 MO-injected (n = 139), 25-N+p53 control MO-injected (n = 351), and flncb+p53 MO-injected (n = 164); †p = 1.65 × 10-19 (for comparison of uninjected embryo vs. flncb+p53 MO using chi-square testing). (B) Representative zebrafish heart pictures for embryo uninjected and flncb MO coinjected with p53 MO zebrafish embryos at 48 hpf. The flncb+p53 MO-injected zebrafish show cardiac phenotype (pericardial edema [P]), and GFP hearts showing less heart looping, elongated atrium, pericardial edema, and a truncated ventricle. (C) Representative zebrafish heart pictures for embryo uninjected and flncb MO coinjected with p53 MO zebrafish embryos at 72 hpf. Among the flncb+p53 MO-injected zebrafish, they show cardiac phenotype, and GFP hearts further show less heart looping, elongated atrium, pericardial edema, and a truncated ventricle.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-flncb + MO4-tp53 standard conditions Long-pec pericardium edematous, abnormal
Protruding-mouth pericardium edematous, abnormal
Days 7-13 whole organism dead, abnormal
Days 7-13 whole organism decreased life span, abnormal
f1Tg + MO2-flncb + MO4-tp53 standard conditions Long-pec atrium elongated, abnormal
Long-pec cardiac ventricle truncated, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec pericardium edematous, abnormal
Protruding-mouth atrium elongated, abnormal
Protruding-mouth cardiac ventricle truncated, abnormal
Protruding-mouth heart looping disrupted, abnormal
Protruding-mouth pericardium edematous, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ JACC Basic Transl Sci