FIGURE

Fig. 1

ID

ZDB-FIG-161220-15

Publication

Shen et al., 2016 - Functional antagonism of alpha-subunits of Kv channel in developing brain ventricular system

Other Figures

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Fig. 1

Insertion of the GBT cassette into the second intron of zebrafish kcng4b causes a mutation. (A-D) Homozygous mutants show a clump of cells in the third ventricle (30 hpf, B,D,D′, white arrow) and hydrocephalus (48 hpf, F,G, black arrow and arrowheads). (H) TAIL-PCR mapped the insertion at position 57,917,547 on Chr. 7 in the (+) orientation (Zv9). (I) Insertion into the kcng4b locus truncates the full-length transcript. kcng4b^−/− expresses the truncated transcript-kcng4b* consisting of one coding exon. (J) RT-PCR analysis demonstrates a reduction of kcng4b transcripts in kcng4b^+/− and their absence in kcng4b^−/− (30 hpf). Actin was detected as a loading control. (A,B) Frontal views, (C-G) lateral views, anterior to the left. E, exon; wt, wild type; A and B, primers as detailed in the Materials and Methods.

Expression Data

Gene:	kcng4b
Fish:	kcng4b^{sq300Gt/sq300Gt} kcng4b^sq300Gt/+
Anatomical Term:	whole organism
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kcng4b^{sq300Gt/sq300Gt} kcng4b^sq300Gt/+
Observed In:	brain heart third ventricle cell whole organism
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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