ZFIN ID: ZDB-FIG-161208-11
Topczewska et al., 2016 - The Morphogenesis of Cranial Sutures in Zebrafish. PLoS One   11:e0165775 Full text @ PLoS One
Anatomical Terms:
Stage: Adult

Fig. 7

RNAscope in situ hybridization and IHC for GFP performed for adults at 12 wpf.

(A-F) Sequential sections (4 μm) of interfrontal suture expression. Names of genes tested by RNAscope in situ hybridization are labeled on each image. (A, B) Representative images of the skull taken at low magnification (5x) that visualize entire expression pattern of (A) col1a1a and (B) col10a1a. (C-F) Images of interfrontal sutures and the expression of representative genes as labeled on each image. (G) Immunohistochemistry for sp7 observed in the interfrontal suture using a GFP reporter in the Tg(sp7:EGFP)b1212 transgenic line. Transverse paraffin section, GFP positive cells (green) and nuclei stained with DAPI (blue).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bglap AB standard conditions Adult inter-frontal joint ISH
Adult osteoblast ISH
col1a1a AB standard conditions Adult inter-frontal joint ISH
Adult mesenchymal cell ISH
Adult osteoblast ISH
col2a1a AB standard conditions Adult Not Detected inter-frontal joint ISH
col10a1a AB standard conditions Adult inter-frontal joint ISH
Adult osteoblast ISH
EGFP Ab12-GFP b1212Tg standard conditions Adult inter-frontal joint IHC
Antibody Labeling Details No data available
Phenotype Details No data available
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One