ZFIN ID: ZDB-FIG-161201-24
Monroe et al., 2016 - Hearing sensitivity differs between zebrafish lines used in auditory research. Hearing Research   341:220-231 Full text @ Hear. Res.
Observed In:
Stage: Adult

Fig. 4

Hair bundle density in the zebrafish saccule. (A) Schematic of the saccular epithelium showing the location of our five sampling regions along the rostral-caudal axis. The confocal image on the right is an example of a phalloidin-labeled saccule. All boxes are 50 × 50 μm and are drawn to scale. V = ventral, R = rostral. (B) Comparison of bundle density in *AB fish and transgenic lines on a *AB background. Hair bundle density significantly differs by genotype and saccular location (2-way ANOVA, F5,225 = 6.88, p < 0.001; and F4,225 = 79.06, p < 0.001, respectively). Bonferroni-corrected posthoc tests were then used to compare each transgenic line to the *AB wildtype fish, where **p < 0.01 indicates significant differences from *AB at that saccular location. (C) There is no significant difference in saccular hair bundle density between wildtype strains (2-way ANOVA, F2,96 = 2.65, p = 0.07). (D) Saccular bundle density does not differ between transgenic ET4 fish and wildtype TL fish (2-way ANOVA, F1,31 = 0.08, p = 0.78). Data are presented as mean ± S.E. N = 4–12 fish per group.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg (AB) standard conditions Adult hair cell anterior macula actin filament bundle of stereocilium decondensed, abnormal
w186Tg (AB) standard conditions Adult hair cell posterior macula actin filament bundle of stereocilium condensed, abnormal
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Reprinted from Hearing Research, 341, Monroe, J.D., Manning, D.P., Uribe, P.M., Bhandiwad, A., Sisneros, J.A., Smith, M.E., Coffin, A.B., Hearing sensitivity differs between zebrafish lines used in auditory research, 220-231, Copyright (2016) with permission from Elsevier. Full text @ Hear. Res.