Fig. 5

Expression behavior and mRNA localization of candidate genes modulated by Fsh in zebrafish testis. The mRNA abundance of selected genes identified using RNAseq approach was investigated by qPCR in testes cultured for 48 h in the absence or presence of Fsh (100 ng/mL) and co-incubated (A) or not (B) with 25 µg/mL trilostane (Tril.), as well as in testes collected 4 h after intraperitoneal (i.p.) injection of Fsh (100 ng/g body weight) (C). In addition, the expression of 2 retinoic acid (RA)-related enzymes was analyzed (separated by a vertical line). Adult males considered for this set of experiments were not pre-exposed to E₂in vivo. Data are shown as mean of fold change ± SEM (n = 7-12) and expressed relative to the control condition, which is set at 1 (dashed line). Asterisks indicate significant differences between treated and control groups (P < 0.05). (D-H)In situ localization of candidate mRNA expression in zebrafish testis. Representative types of germ cell cysts are encircled and labeled. No specific staining was observed with sense cRNA probes (insets). A_und, type A undifferentiated spermatogonia; A_diff, type A differentiating spermatogonia; B, type B spermatogonia; SPC, spermatocytes; SPT, spermatids (indicated by an inner asterisk in panel H). Scale bar represents 25 µm.