FIGURE

Fig. 7

ID
ZDB-FIG-160914-31
Publication
Pantoja et al., 2016 - Neuromodulatory Regulation of Behavioral Individuality in Zebrafish
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Fig. 7

Effect on ASR Habituation of DRN Activation with Channelrhodopsin-2

(A) Representative image showing channelrhodopsin-2 (ChR2)-YFP expression in cell bodies and processes of DRN neurons in tg(tph2:Gal4ff; 14xUAS-E1b:hChR2(H134R)-EYFP) in 6 dpf larva.

(B) Protocol showing single stimulus from habituation series, pairing illumination at 488 nm (to activate ChR2 in DRN neurons) with sound stimulation (to elicit the ASR).

(C and D) Acoustic startle responses of all of the individual larvae ordered in the y axis by probability of escape during the stimulus series, separated into two groups from the sibling larvae: not expressing ChR2-YFP (C, Control) and those expressing ChR2-YFP (D, ChR2). Each dot represents a sound-induced escape.

(E) Plots of mean ± SEM of data shown in (C) and (D) showing less habituation in siblings with ChR2 in DRN neurons (nChR2 = 18, nControl = 18, analysis of covariance, F-statistic: 28.24, p < 10-6).
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron
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