ZFIN Image: Pantoja et al., 2016, Fig. 7

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Figure Caption

Fig. 7

Effect on ASR Habituation of DRN Activation with Channelrhodopsin-2

(A) Representative image showing channelrhodopsin-2 (ChR2)-YFP expression in cell bodies and processes of DRN neurons in tg(tph2:Gal4ff; 14xUAS-E1b:hChR2(H134R)-EYFP) in 6 dpf larva.

(B) Protocol showing single stimulus from habituation series, pairing illumination at 488 nm (to activate ChR2 in DRN neurons) with sound stimulation (to elicit the ASR).

(C and D) Acoustic startle responses of all of the individual larvae ordered in the y axis by probability of escape during the stimulus series, separated into two groups from the sibling larvae: not expressing ChR2-YFP (C, Control) and those expressing ChR2-YFP (D, ChR2). Each dot represents a sound-induced escape.

(E) Plots of mean ± SEM of data shown in (C) and (D) showing less habituation in siblings with ChR2 in DRN neurons (n_ChR2 = 18, n_Control = 18, analysis of covariance, F-statistic: 28.24, p < 10^-6).

Acknowledgments

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