FIGURE

Fig. 3

ID
ZDB-FIG-160909-3
Publication
Noonan et al., 2016 - Loss of vhl in the zebrafish pronephros recapitulates early stages of human clear cell renal cell carcinoma
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Vhl-/- larvae have an increased amount of lipids in the proximal pronephros compared to wt siblings. (A-D) BODIPY 493/503 immunofluorescence was performed in live whole-mount 5.5dpf vhl-/- and wt siblings. BODIPY 493/503 staining (in green) intensity was then quantified within a ROI encompassing the proximal pronephros (yellow box), using ImageJ. Confocal images of BODIPY staining in vhl-/- (C) versus wt sibling (A). Confocal images of BODIPY staining in vhl-/- (D) versus wt sibling (B). (E) Quantification of mean BODIPY 493/503 staining intensity within the ROI in 5.5dpf vhl-/- versus wt siblings. Each experiment had at least three larvae per sample group and was performed in triplicate. Data represent meanĀ±s.e.m. ****P<0.0001 (paired two-tailed t-test).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.