ZFIN ID: ZDB-FIG-160708-5
He et al., 2016 - Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain. eLIFE   5 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: Days 21-29 to Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 21-29 to Days 30-44

Fig. 4

Loss of nf1 promotes neuroblastoma tumor progression in MYCN transgenic fish.

(A) Neuroblastoma development in representative fish of the nf1a+/+;nf1b+/+;MYCN;GFP, nf1a+/-;nf1b+/+;MYCN;GFP and nf1a-/-;nf1b+/+;MYCN;GFP genotypes over 3 to 6 weeks of age.Each fish was imaged weekly for 3 continuous weeks from 3 weeks of age. (B) Quantification of GFP+ sympathoadrenal cells in the IRG of fish with nf1a+/+;nf1b+/+;MYCN;GFP (n = 10), nf1a+/-;nf1b+/+;MYCN;GFP (n = 21) and nf1a-/-;nf1b+/+;MYCN;GFP (n = 15) genotypes, demonstrating tumor progression over 3 weeks. Tumors were scored when the GFP+ signals in the IRG exceeded the threshold defined by the dotted line. (C) IRG development in nf1a mutant zebrafish lacking overexpression of MYCN. Quantification of GFP+ sympathoadrenal cells in the IRG of fish with nf1a+/+;nf1b+/+;GFP (n = 10), nf1a+/-;nf1b+/+;GFP (n = 10) and nf1a-/-;nf1b+/+;GFP (n = 10) genotypes. The same tumor threshold line shown in panel B is included for comparison.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nf1ap301/+; zdf16Tg standard conditions Days 21-29 to Days 30-44 interrenal gland neuroblastoma IFL
nf1ap301/p301; zdf16Tg standard conditions Days 21-29 to Days 30-44 interrenal gland neuroblastoma IFL
zdf16Tg standard conditions Days 21-29 to Days 30-44 interrenal gland neuroblastoma IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nf1ap301/+; zdf16Tg standard conditions Days 21-29 - Days 30-44 interrenal gland neuroblastoma increased occurrence, exacerbated
nf1ap301/p301; zdf16Tg standard conditions Days 21-29 - Days 30-44 interrenal gland neuroblastoma increased occurrence, exacerbated
zdf16Tg standard conditions Days 21-29 - Days 30-44 interrenal gland neuroblastoma increased occurrence, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife