ZFIN ID: ZDB-FIG-160708-2
He et al., 2016 - Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain. eLIFE   5 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 21-29 to Adult

Fig. 2

Loss of nf1 accelerates disease onset and increases the penetrance of MYCN-induced neuroblastoma.

(A) Representative fish of nf1a+/+;nf1b+/+;GFP, nf1a+/+;nf1b+/+;MYCN;GFP, nf1a-/-;nf1b+/+;GFP and nf1a-/-;nf1b+/+;MYCN;GFP genotypes at 5 weeks of age.The interrenal glands (IRGs) are highlighted with dashed circles. (B) H&E-stained sagittal sections of a 10-week old nf1a+/+;nf1b+/+;GFP fish (left) and a 10-week old nf1a-/-;nf1b+/-;GFP;MYCN fish (middle) and 63-fold magnified tumor cells (right), which are magnified from the area in the small white box in the middle panel. (C) Cumulative frequency of neuroblastoma in MYCN transgenic zebrafish representing all eight nf1 genotypes generated by the breeding of the nf1a+/-;nf1b+/-;MYCN line with the nf1a+/-;nf1b+/- zebrafish line (*p<0.0001 nf1a-/-;nf1b+/+ vs. nf1a+/+;nf1b+/+).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nf1ap301/p301; zdf15Tg standard conditions Days 30-44 superior cervical ganglion IFL
nf1ap301/p301; zdf16Tg standard conditions Days 30-44 superior cervical ganglion IFL
zdf15Tg standard conditions Days 30-44 superior cervical ganglion IFL
zdf16Tg standard conditions Days 30-44 superior cervical ganglion IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nf1ap301/+; nf1bp304/+; zdf16Tg standard conditions Days 21-29 - Adult interrenal gland neuroblastoma increased occurrence, exacerbated
nf1ap301/+; nf1bp304/p304; zdf16Tg standard conditions Days 21-29 - Adult interrenal gland neuroblastoma increased occurrence, exacerbated
nf1ap301/+; zdf16Tg standard conditions Days 21-29 - Adult interrenal gland neuroblastoma increased occurrence, exacerbated
nf1ap301/p301; nf1bp304/+; zdf16Tg standard conditions Days 21-29 - Adult interrenal gland neuroblastoma increased occurrence, exacerbated
nf1ap301/p301; zdf16Tg standard conditions Days 21-29 - Adult interrenal gland neuroblastoma increased occurrence, exacerbated
Days 30-44 interrenal gland neuroblastoma increased occurrence, exacerbated
nf1bp304/+; zdf16Tg standard conditions Days 21-29 - Adult interrenal gland neuroblastoma increased occurrence, abnormal
nf1bp304/p304; zdf16Tg standard conditions Days 21-29 - Adult interrenal gland neuroblastoma increased occurrence, abnormal
zdf16Tg standard conditions Days 21-29 - Adult interrenal gland neuroblastoma increased occurrence, abnormal
Days 30-44 interrenal gland neuroblastoma increased occurrence, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife