FIGURE

Fig. 6

ID

ZDB-FIG-160602-26

Publication

Steventon et al., 2016 - Species tailoured contribution of volumetric growth and tissue convergence to posterior body elongation in vertebrates

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Fig. 6

FGF signalling is required for thinning and lengthening, but not volumetric growth of the zebrafish posterior body. (A-D) Embryos injected at the one-cell stage with nuclear mCherry mRNA and incubated in either 500µM DMSO (A), 200µM SU5402 (B), 500µM SU5402 (C) or 20µM PD173074 (D) from the ten-somite stage until the 32-somite stage and imaged by confocal microscopy at the 32-somite stage. (E-G) Mean value of posterior body volume (E), length (F) and width (G) for each treatment group at the 32-somite stage. Error bars indicate s.d. (H,I) Stills from time-lapse movies of embryos in which small regions within the tailbud are photolabelled at the 12-somite stage in the presence of 500µM DMSO (H) or 20µM PD173074 (I). (J,K) Quantification of the volume (J) and length:width ratio (K) of the photolabelled region over time (number of somites). Green lines correspond to the control situation (500µM DMSO), red lines indicate treatment with PD173074.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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