Fig. S4

Knockdown of klf8 does not affect cell proliferation or apoptosis in the trunk muscle. (A-G, I-O) Lateral views of embryos and myotomes above the yolk extension are shown. The number of pH3-expressing M phase cells in myotomes at 24 hpf were similar between WT embryos (A), embryos injected with klf8-4mmMO1 (B), klf8-MO1atg (C), klf8-MO2atg (D), or p53 MOsp (G), and embryos co-injected with klf8-MO^AC and klf8-MO^DO (E), or p53 MOsp and klf8-MO1^atg (F). (H) Mitotic indices of embryos of the indicated backgrounds. The numbers of TUNEL-positive cells in the myotomes at 24 hpf were similar between WT embryos (I), embryos injected with klf8-4mmMO1 (J), klf8-MO1^atg (K), klf8-MO2^atg (L), or p53 MO^sp (O), and embryos co-injected with klf8-MOAC and klf8-MODO (M), or p53 MOsp and klf8-MO1atg (N). (P) Percentage of TUNEL-positive cells in embryos of the indicated backgrounds. Error bars indicate standard errors.