FIGURE

Fig. 1

ID

ZDB-FIG-151009-12

Publication

Rydeen et al., 2015 - Excessive feedback of Cyp26a1 promotes cell non-autonomous loss of retinoic acid signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

VP-RAR promotes a prolonged increase in cyp26a1 expression. (A–H) Wholemount ISH shows a dramatic increase in cyp26a1 expression in vp-rar mRNA injected embryos compared to WT siblings at 30% epiboly through 14 s stage. (I–P) ISH of RA synthesizing genes rdh10a and aldh1a2 shows modestly decreased expression in vp-rar mRNA injected embryos compared to WT at the shield stage (I,J,M,N), with dramatic decreases found by the tailbud stage (K,L,O,P). (Q,R) ISH using a six6b (anterior diencephalon), eng2a (midbrain–hindbrain boundary), egr2b (rhombomeres 3 and 5), and myod1 (adaxial cells and somites) cocktail (referred to here at sekm cktl) shows a lack of eng2a and severely reduced six6b and rhombomere 3 (anterior egr2b) in vp-rar mRNA injected compared to WT. Arrows in R indicate the loss of eng2a and reduced rhombomere 3. (S,T) Expression of hoxb5b is decreased in the lateral plate mesoderm (brackets) and anterior spinal cord (arrows) in vp-rar mRNA injected embryos compared to WT siblings. (U) RT-qPCR with cyp26a1, rdh10a, aldh1a2, and hoxb5b expression in vp-rar mRNA injected embryos at the shield (sh) through 24 s stages. Asterisks indicate statistically different from control (p<0.05) using Student′s t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 405(1), Rydeen, A., Voisin, N., D'Aniello, E., Ravisankar, P., Devignes, C.S., Waxman, J.S., Excessive feedback of Cyp26a1 promotes cell non-autonomous loss of retinoic acid signaling, 47-55, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.