FIGURE

Fig. 6

ID

ZDB-FIG-150924-13

Publication

Seiler et al., 2015 - Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish

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Fig. 6

Morpholino knockdown of agtpbp1 and eps15L1 results in decreased lymphoid rag1 expression.

(A-B) WISH of rag1 in 4 dpf control (A) and agtpbp1 (B) morpholino-injected embryos. (C) RT-PCR of agtpbp1 and β-actin in pooled control or morphant embryo samples. Quantitation of the normal transcript band normalized to ß-actin is indicated. (D-E) WISH of rag1 in 4 dpf control (A) and eps15L1 (B) morpholino-injected embryos. (C) RT-PCR of eps15L1 and ß-actin in pooled control or morphant embryo samples. Quantitation of the normal transcript band normalized to β-actin is indicated. Quantitation is in arbitrary units (A.U.), and relative to wild-type level which is set at 1. Head region of the embryos is shown in lateral views, anterior to the left. P values were determined using Fisher’s exact test.

Expression Data

Gene:	rag1
Fish:	WT WT + MO1-eps15l1b + MO4-tp53 WT + MO3-agtpbp1 + MO4-tp53
Knockdown Reagents:	MO1-eps15l1b MO3-agtpbp1 MO4-tp53
Anatomical Term:	thymus
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-eps15l1b + MO4-tp53 WT + MO3-agtpbp1 + MO4-tp53
Knockdown Reagents:	MO1-eps15l1b MO3-agtpbp1 MO4-tp53
Observed In:	T cell differentiation in thymus thymus
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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