FIGURE

Fig. 1

ID

ZDB-FIG-150923-1

Publication

Helmprobst et al., 2015 - Presynaptic architecture of the larval zebrafish neuromuscular junction

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Fig. 1

Differences in ultrastructural preservation in chemically fixed and cryoimmobilized and freeze substituted samples. The overview shows electron micrographs of a chemically fixed (A) and an HPF/FS-treated (E) synapse (S) of a ~250-nm-thick section from a 4-dpf zebrafish larva. Synapses are surrounded by muscle cells (M) with an electron-dense synaptic cleft (white arrows) in between (B,C,F,G). C and G show a tomogram slice per synapse. Mitochondria (asterisks) and synaptic vesicles (black arrows) are annotated. A higher magnification image of the boxed region in C and G is shown in D and H, respectively. Scale bars = 1 µm A,E; 500 nm in B,F; 200 nm in C,G; 100 nm in D,H.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.