FIGURE

Fig. 6

ID
ZDB-FIG-150526-12
Publication
Petersen et al., 2015 - The adhesion GPCR GPR126 has distinct, domain-dependent functions in Schwann cell development mediated by interaction with laminin-211
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Fig. 6

Laminin-211 Activates Gpr126 under Dynamic Conditions In Vitro and Polymerizing Conditions In Vivo

(A and B) COS-7 cells were transiently transfected with empty vector (eV) and hGPR126 plasmid. cAMP accumulation was measured after stimulation with Laminin-211 (200 ng/ml) and the indicated force. Data are represented as mean ± SEM of three independent assays each performed in triplicate. p < 0.05, p < 0.01, p < 0.001, two-way ANOVA with Tukey’s multiple comparisons test (each mechanic-induced response was compared to static conditions for all hGPR126 data points, with and without Laminin-211).

(A) Laminin-211 suppresses GPR126 signaling under stationary conditions (Hz 0) but causes a frequency-dependent increase of cAMP accumulation with increasing vibration.

(B) Mechanical stimulation via shaking further enhances Laminin-211-dependent cAMP accumulation with increasing frequency.

(C–H) TEM of 5 dpf zebrafish PLLn. Myelinated axons are pseudocolored in green. The scale bar represents 1 µm.

(C–E) Myelination proceeds normally in a control larva (C), in a larva injected with 40 pg WT lama2 OE construct (D), and in a larva injected with 40 pg mutant lama2(dy2j) OE construct (E).

(F) Myelination is reduced in a control-injected gpr126st63/st63 larva.

(G) Injection of 40 pg lama2 OE rescues myelination in a gpr126st63/st63 larva.

(H) Injection of 40 pg lama2(dy2j) OE fails to rescue myelination in a gpr126st63/st63 larva.

(I–K) Quantification of TEM images. Bars represent means ± SD. p < 0.01, p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.

(I) Number of sorted axons per PLLn.

(J) Number of myelinated axons per PLLn.

(K) Number of total axons per PLLn.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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