FIGURE

Fig. 6

ID

ZDB-FIG-150330-10

Publication

Marín-Juez et al., 2015 - GLUT2-mediated glucose uptake and availability are required for embryonic brain development in zebrafish

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Fig. 6

Abrogation of glut2 enhances cell death mainly in the head region. To assay for cell death, embryos injected with control morpholino (Con MO; A), ATG morpholino (ATG MO; B and C), and ATG MO+rat GLUT2 mRNA (ATG MO Rescued; D) were stained with the vital dye acridine orange. To assay for apoptosis, embryos injected with Con MO (E) and ATG MO (F) were analyzed by TUNEL assay. At 24 hpf, there was an overall increase in cell death and apoptosis primarily localized in the hindbrain region (B, C, and F). Fluorescent signal analysis (G) and counting of TUNEL-positive cells (H) confirmed a significant increase in cell death and apoptosis, respectively, in ATG morphants which appears reverted in rescued embryos (G). * indicates significant differences compared with the Con MO injected embryos (*P<0.01; ***P<0.001). Hindbrain ventricle (hbv), midbrain/hindbrain boundary (mbh), telencephalon (t). In the images (A–F), the position of the embryos is indicated by a representation of a zebrafish embryo.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL + MO1-slc2a2
Knockdown Reagent:	MO1-slc2a2
Observed In:	hindbrain apoptotic process
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cereb. Blood Flow Metab.