ZFIN Figure: Dozawa et al., 2014, Fig. 2

ZFIN ID: ZDB-FIG-150326-37

ZFIN ID: ZDB-FIG-150326-37

Dozawa et al., 2014 - Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum. Developmental dynamics : an official publication of the American Association of Anatomists 243(11):1401-15 Full text @ Dev. Dyn.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	EGFP neurod1
Fish:	WT knu3Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	hindbrain neuron
Stage:	Long-pec

PHENOTYPE:

Fish:	WT
Condition:	chemical treatment: pharmaceutical
Observed In:	hindbrain cell population proliferation hindbrain neurogenesis
Stage:	Long-pec

Fig. 2

The HDAC inhibitor TSA mimics the effect of VPA on cell proliferation in embryonic zebrafish. A: TSA-treated brains were stained with anti-BrdU antibody. Number of BrdU-positive cells increased in a dose-dependent manner, and the area of BrdU-positive cells expanded in the TSA-treated brain. B: Number of BrdU-positive cells per section. C: Tg(elavl3:GFP) fish were treated with TSA. GFP-positive area decreased in the TSA-treated embryo, similar to the VPA-treated embryo. D: In situ hybridization of 400 nM TSA-treated embryos with neurod probe. Expression of neurod decreased in the TSA-treated brain. E: Acetylated histone H4 (Ac-H4) level was analyzed by Western blot analysis in brain homogenates from zebrafish embryos treated with indicated drugs. Increased Ac-H4 level was observed in 1.0 mM VPA and 400 nM TSA-treated samples. **P < 0.01, ***P < 0.001

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	knu3Tg	control	Long-pec	hindbrain neuron	IHC
	knu3Tg	chemical treatment: pharmaceutical	Long-pec	hindbrain neuron	IHC
neurod1	WT	control	Long-pec	hindbrain neuron	ISH
	WT	chemical treatment: pharmaceutical	Long-pec	hindbrain neuron	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT	chemical treatment: pharmaceutical	Long-pec	hindbrain cell population proliferation increased process quality, abnormal
		Long-pec	hindbrain neurogenesis decreased process quality, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.