ZFIN ID: ZDB-FIG-150326-37 |
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Stage: | Long-pec |
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Stage: | Long-pec |
Fig. 2 The HDAC inhibitor TSA mimics the effect of VPA on cell proliferation in embryonic zebrafish. A: TSA-treated brains were stained with anti-BrdU antibody. Number of BrdU-positive cells increased in a dose-dependent manner, and the area of BrdU-positive cells expanded in the TSA-treated brain. B: Number of BrdU-positive cells per section. C: Tg(elavl3:GFP) fish were treated with TSA. GFP-positive area decreased in the TSA-treated embryo, similar to the VPA-treated embryo. D: In situ hybridization of 400 nM TSA-treated embryos with neurod probe. Expression of neurod decreased in the TSA-treated brain. E: Acetylated histone H4 (Ac-H4) level was analyzed by Western blot analysis in brain homogenates from zebrafish embryos treated with indicated drugs. Increased Ac-H4 level was observed in 1.0 mM VPA and 400 nM TSA-treated samples. **P < 0.01, ***P < 0.001 |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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EGFP | knu3Tg | control | Long-pec | hindbrain neuron | IHC | |
knu3Tg | chemical treatment: pharmaceutical | Long-pec | hindbrain neuron | IHC | ||
neurod1 | WT | control | Long-pec | hindbrain neuron | ISH | |
WT | chemical treatment: pharmaceutical | Long-pec | hindbrain neuron | ISH |
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