ZFIN ID: ZDB-FIG-150326-36
Dozawa et al., 2014 - Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum. Developmental dynamics : an official publication of the American Association of Anatomists   243(11):1401-15 Full text @ Dev. Dyn.
Anatomical Term:
Stage: Long-pec
Observed In:
Stage: Long-pec

Fig. 1

VPA increases proliferating cells in the hindbrain of embryonic zebrafish. A: Embryonic zebrafish were treated with 1.0, 1.5, 2.0 mM VPA from 10 to 48 hpf. In last 20 min, BrdU was added to label proliferating cells. B: Number of BrdU-positive cells increased in a dose-dependent manner, and the area of BrdU-positive cells expanded in the VPA-treated brain. C: Number of BrdU-positive cells per section. D: Tg(elavl3:GFP) and Tg(gfap:GFP) were treated with VPA at indicated concentrations, and the GFP-positive area was analyzed. Reduction of elavl3-GFP-positive area (a-e, a′-e′) and increase of gfap-GFP-positive area (f-j, f′-j′) were observed in VPA-treated zebrafish hindbrain. E: In situ hybridization of VPA-treated embryonic brain with neurod probes. Area of neurod-positive decreased in VPA-treated zebrafish hindbrain. After whole-mount in situ hybridization, coronal sections were made at rhombomere 5 or 6 of the hindbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg control Long-pec hindbrain neuron IHC
knu3Tg chemical treatment: pharmaceutical Long-pec hindbrain neuron IHC
GFP mi2001Tg control Long-pec hindbrain neuron IHC
mi2001Tg chemical treatment: pharmaceutical Long-pec hindbrain neuron IHC
neurod1 WT control Long-pec hindbrain neuron ISH
WT chemical treatment: pharmaceutical Long-pec hindbrain neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical Long-pec hindbrain cell population proliferation increased process quality, abnormal
Long-pec hindbrain neurogenesis decreased process quality, abnormal
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