ZFIN ID: ZDB-FIG-150326-36
Dozawa et al., 2014 - Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum. Developmental dynamics : an official publication of the American Association of Anatomists   243(11):1401-15 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 1

VPA increases proliferating cells in the hindbrain of embryonic zebrafish. A: Embryonic zebrafish were treated with 1.0, 1.5, 2.0 mM VPA from 10 to 48 hpf. In last 20 min, BrdU was added to label proliferating cells. B: Number of BrdU-positive cells increased in a dose-dependent manner, and the area of BrdU-positive cells expanded in the VPA-treated brain. C: Number of BrdU-positive cells per section. D: Tg(elavl3:GFP) and Tg(gfap:GFP) were treated with VPA at indicated concentrations, and the GFP-positive area was analyzed. Reduction of elavl3-GFP-positive area (a-e, a′-e′) and increase of gfap-GFP-positive area (f-j, f′-j′) were observed in VPA-treated zebrafish hindbrain. E: In situ hybridization of VPA-treated embryonic brain with neurod probes. Area of neurod-positive decreased in VPA-treated zebrafish hindbrain. After whole-mount in situ hybridization, coronal sections were made at rhombomere 5 or 6 of the hindbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg control Long-pec hindbrain neuron IHC
knu3Tg chemical treatment: pharmaceutical Long-pec hindbrain neuron IHC
GFP mi2001Tg control Long-pec hindbrain neuron IHC
mi2001Tg chemical treatment: pharmaceutical Long-pec hindbrain neuron IHC
neurod1 WT control Long-pec hindbrain neuron ISH
WT chemical treatment: pharmaceutical Long-pec hindbrain neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical Long-pec hindbrain cell population proliferation increased process quality, abnormal
Long-pec hindbrain neurogenesis decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.