FIGURE

Fig. 5

ID

ZDB-FIG-150326-17

Publication

Li et al., 2014 - Zebrafish nephrogenesis is regulated by interactions between retinoic acid, mecom, and Notch signaling

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Fig. 5

mecom and RA have opposing roles in PST and DL formation during proximodistal segmentation of the pronephros. (A) Wild type embryos or mecom morphants were incubated with 1×10⁷ M RA, 1.6×10⁵ M DEAB or DMSO. WISH using the PST marker trpm7 and DL marker slc12a3 showed that exogenous RA resulted in a more severe segmental phenotype in the mecom morphant pronephros, with further expanded PST and reduced DL. DEAB treatment partially rescued the segmentation phenotype in mecom morphants. (B) Schematic summary of segmentation changes in wild type embryos, mecom morphants, and wild type embryos or morphants treated with RA or DEAB. Yellow and blue boxes highlight the PST and DL segments, respectively.

Expression Data

Genes:	slc12a3 smyhc1 trpm7
Fish:	WT WT + MO3-mecom + MO4-mecom
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO3-mecom MO4-mecom
Anatomical Terms:	pronephric distal late tubule pronephric proximal straight tubule somite
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO3-mecom + MO4-mecom
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO3-mecom MO4-mecom
Observed In:	pronephric distal late tubule pronephric proximal straight tubule
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Li, Y., Cheng, C.N., Verdun, V.A., and Wingert, R.A., Zebrafish nephrogenesis is regulated by interactions between retinoic acid, mecom, and Notch signaling, 111-122, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.