FIGURE

Fig. 3

ID

ZDB-FIG-150326-15

Publication

Li et al., 2014 - Zebrafish nephrogenesis is regulated by interactions between retinoic acid, mecom, and Notch signaling

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Fig. 3

mecom knockdown leads to an expanded PST segment. (A) At 24 hpf, WISH indicates proximal segments marked by slc4a4a elaborated in mecom morphants. Within proximal domains, PCT labeled by slc20a1a was not affected compared with wild type embryos. Knockdown of mecom induced a 3-somite expansion in trpm7-expressing PST in morphant pronephros at 24 hpf, which could be rescued by co-injecting mecom cRNA along with mecom morpholinos. (B) Schematic summary of proximal segment organization in wild type, mecom morphant, and mecom rescued embryos, with PST alterations highlighted in yellow. Abbreviations: PCT (proximal convoluted tubule), and PST (proximal straight tubule).

Expression Data

Genes:	slc4a4a slc20a1a smyhc1 trpm7
Fish:	WT WT + MO3-mecom + MO4-mecom
Knockdown Reagents:	MO3-mecom MO4-mecom
Anatomical Terms:	pronephric proximal convoluted tubule pronephric proximal straight tubule somite
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-mecom + MO4-mecom
Knockdown Reagents:	MO3-mecom MO4-mecom
Observed In:	pronephric proximal straight tubule
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Li, Y., Cheng, C.N., Verdun, V.A., and Wingert, R.A., Zebrafish nephrogenesis is regulated by interactions between retinoic acid, mecom, and Notch signaling, 111-122, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.