ZFIN ID: ZDB-FIG-140902-104
Pinzón-Olejua et al., 2014 - Essential roles of zebrafish rtn4/Nogo paralogues in embryonic development. Neural Development   9:8 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 7

Brain differentiation and retinotectal projections in rtn4a- and rtn4b-knockdown, GFP–transgenic embryos. In rtn4a-morpholino (MO)- and rtn4b-MO-injected embryos, brains are smaller both anteroposteriorly as well as laterally. (A) Extent of the tectum (t) and the forebrain (fb) marked by white brackets in the HuC/HuD labeled brains of control embryos. (D) and (G) Knockdown of rtn4a, but more severely of rtn4b, leads to a reduction in the size of the tectum and the forebrain. In rtn4b morphants, the olfactory placodes (arrowhead) are not clearly identifiable and the tectum is localized in abnormally anterior positions. (B) In control embryos, the GFP–labeled retinal ganglion cell (RGC) axons cover the tectal neuropil (outlined). (E) and (H) The RGC axons in the tectum cover a smaller area in both morphants, but more severely so in the rtn4b morphants than in their rtn4a counterparts. GFP is also expressed in neuromasts (arrows), which are aberrantly positioned in the morphants. (C), (F) and (I) 42,6-diamidino-2-phenylindole (DAPI) staining of the brain shows the area of the neuropil (outlined), which is reduced after downregulation of rtn4a and rtn4b. (J), (K) and (L) Quantification of the length of the optic tectum, forebrain and tectal neuropil in control and rtn4a-MO- and rtn4b-MO-injected embryos. (A) to (I) Dorsal views of the brain of tg(brn3c:mGFP) embryos at 5 dpf. Control MO (5.0 ng) (n = 10), rtn4a-l-MO1 (5.0 ng) (n = 10) and rtn4b-MO1 (5.0 ng) (n = 10). Scale bar = 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s356tTg control Day 5 neuromast IFL
Day 5 retinal ganglion cell axon IFL
s356tTg + MO2-rtn4b standard conditions Day 5 neuromast IFL
Day 5 retinal ganglion cell axon IFL
s356tTg + MO9-rtn4a standard conditions Day 5 neuromast IFL
Day 5 retinal ganglion cell axon IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT control Day 5 brain neuron
IHC Day 5 forebrain neuron
IHC Day 5 optic tectum neuron
IHC WT + MO2-rtn4b standard conditions Day 5 brain neuron
IHC Day 5 forebrain neuron
IHC Day 5 optic tectum neuron
IHC WT + MO9-rtn4a standard conditions Day 5 brain neuron
IHC Day 5 forebrain neuron
IHC Day 5 optic tectum neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-rtn4b standard conditions Day 5 brain decreased size, abnormal
Day 5 forebrain decreased size, abnormal
Day 5 olfactory placode morphology, abnormal
Day 5 optic tectum decreased size, abnormal
Day 5 optic tectum has fewer parts of type retinal ganglion cell neuron projection, abnormal
Day 5 optic tectum mislocalised anteriorly, abnormal
WT + MO9-rtn4a standard conditions Day 5 brain decreased size, abnormal
Day 5 forebrain decreased size, abnormal
Day 5 optic tectum decreased size, abnormal
s356tTg + MO2-rtn4b standard conditions Day 5 head altered number of neuromast, abnormal
Day 5 neuromast displaced, abnormal
Day 5 optic tectum has fewer parts of type retinal ganglion cell neuron projection, abnormal
s356tTg + MO9-rtn4a standard conditions Day 5 head altered number of neuromast, abnormal
Day 5 neuromast displaced, abnormal
Day 5 optic tectum has fewer parts of type retinal ganglion cell neuron projection, abnormal
Acknowledgments:
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