ZFIN ID: ZDB-FIG-140818-21
Zhong et al., 2014 - Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis. Cell death and differentiation   21:1013-24 Full text @ Cell Death Differ.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to Bud

Fig. 4

The ability of zNoxa is suppressed by zMcl1a and zMcl1b. (a) Expression of zNoxa in WT and p53 MO-injected embryos was evaluated by real-time PCR and normalized to β-actin mRNA. (b) pcDNA3.1-HA-zMcl1a and pcDNA3.1-HA-zMcl1b were injected into zebrafish embryos to assess an interaction with endogenous zNoxa. At shield stage, zMcl1a-HA and zMcl1b-HA were immunoprecipitated (IP) and then the co-precipitation of zNoxa was assessed by immunobloting. (c) Confocal z-stacks of mitotic cells assessed by pH3 staining in embryos which were WT (i) and injected with zNoxa mRNA (ii), combined zNoxa mRNA and zMcl1a&b mRNA (iii), zMcl1a&b MOs (iv), zNoxa MO1+2 plus zMcl1a&b MOs (v) or mNoxa mRNA together with zMcl1a&b mRNA (vi) at shield stage. Embryos stained by pH3 were lateral views with animal pole toward the top and dorsal to the right. (d) Quantification of numbers of pH3-positive cells with five embryos for each group in (c). Error bars represent S.E.M. (n=5). (e) A typical FACS analysis of PI-labeled cells in embryos for each group in c. (f) The FACS statistical data of three independent experiments in e. Error bars represent S.E.M. (n=3). (g) Confocal z-stacks of apoptotic cells assessed by TUNEL in embryos which were WT (i), injected with zNoxa mRNA (ii), combined zNoxa mRNA and zMcl1a&b mRNA (iii), zMcl1a&b MO (iv), zNoxa MO1+2 together with zMcl1a&b MO (v) at bud stage. Embryos were lateral views with animal pole toward the top and dorsal to the right. (h) Quantification of numbers of TUNEL-positive cells with four embryos for each group in g. Error bars represent S.E.M. (n=4)

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mcl1a + MO1-mcl1b standard conditions Shield whole organism cell population proliferation increased process quality, abnormal
Shield whole organism mitotic cell cycle process quality, abnormal
Bud whole organism apoptotic process increased process quality, abnormal
Bud whole organism cell population proliferation increased process quality, abnormal
Acknowledgments:
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