ZFIN ID: ZDB-FIG-140613-22
Zhong et al., 2014 - Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis. Cell death and differentiation   21:1013-24 Full text @ Cell Death Differ.
Knockdown Reagents:
Anatomical Terms:
Stage Range: 8-cell to Bud

Fig. 5 Activities of zWnt4b is regulated by zNoxa. (a) Expression of zWnt4b in embryos, which were WT (i) and injected with zNoxa MO1+2 (ii), zNoxa mRNA (iii), zNoxa MO1+2 plus zNoxa rcRNA (iv), mNoxa mRNA (v), together mNoxa mRNA with zNoxa mRNA (vi), zMcl1a&b mRNA (vii), or zMcl1a&b MO (viii), was detected by in situ hybridization. (b) Expression of zWnt4b in embryos for each group in a. (c) Expression pattern of zWnt4b was detected by in situ hybridization using specific antisense or sense probes. No staining was present in embryos hybridized with sense probe (the shield stage shown). Embryo were lateral views with anterior toward the top. (d) Expression of zWnt4b in embryonic development stages was checked by real-time PCR and normalized to β-actin mRNA. Error bars represent S.E.M. (n=3). (e) Zebrafish embryos were collected at different stages and protein was extracted for immunoblot analysis of zWnt4b and β-actin after discarding the yolk. β-actin served as a loading control

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
wnt4b WT standard conditions 8-cell Not Detected whole organism ISH
High blastomere ISH
High whole organism ISH
Sphere whole organism ISH
Shield whole organism ISH
75%-epiboly whole organism ISH
Bud whole organism ISH
WT + MO1-mcl1a + MO1-mcl1b standard conditions Shield whole organism ISH
WT + MO2-pmaip1 + MO3-pmaip1 standard conditions Shield whole organism ISH
Antibody Labeling Details No data available
Phenotype Details No data available
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