ZFIN Figure: Kelly et al., 2014, Fig. 1

Fig. 1

Pak1 Is Required for Normal Development of Zebrafish

(A) Transcripts for pak1 were detected by whole-mount in situ hybridization at the stages indicated. epib, epiboly.

(B) Expression of pak1 at various stages in development by RT-PCR.

(C) RT-PCR analysis of pak1 transcripts in morphants compared to WT and MM-injected embryos at 24 and 48 hpf.

(D) Immunoblot analysis of Pak1 in morphants compared to WT at 24 and 48 hpf.

(E) Representative images of the moderate (MO moderate) and severe (MO severe) pak1 knockdown phenotypes as compared to the WT and MM-injected embryos at 24 hpf and 48 hpf.

(F) Quantification of pak1 knockdown phenotype at 24 hpf. p < 0.0001.

(G) Morphology of embryos injected with the indicated MOs plus mRNA for WT of kinase-dead human Pak1 (hPak1), WT zebrafish Pak1 (zPak1), zPak2a, or zPak2b.

(H) Quantitation of phenotypes at 24 hpf. p < 0.0001.

Expression Data

Gene:	pak1
Fish:	WT
Anatomical Terms:	central nervous system heart intersegmental vessel whole organism
Stage Range:	1-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-pak1
Knockdown Reagent:	MO2-pak1
Observed In:	blood circulation head cell death heart pericardium portion of tissue whole organism
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 29, Kelly, M.L., Astsaturov, A., Rhodes, J., Chernoff, J., A Pak1/Erk Signaling Module Acts through Gata6 to Regulate Cardiovascular Development in Zebrafish, 350-9, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell