FIGURE

Fig. 10

ID

ZDB-FIG-140617-23

Publication

Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish

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Fig. 10

Endocrine cell differentiation is abolished in the double ascl1b and neurod1 morphants. Ventral views of WISH showing expression of insulin(A-B, I-J), somatostatin(C-D), ghrelin(E-F) and glucagon(G-H) in control (A,C,E,G,I) and Mo-ascl1b/Mo2-neurod1(B,D,F,H,J) morphants at 38 hpf (A-H) or 17 hpf (I-J). The quantifications on the right side of the figure represent the number of positive cells per embryo for controls and double ascl1b/neurod1 morphants. Asterisks (***) indicate that the difference between cell number in controls and double ascl1b/neurod1 morphants is statistically significant by Student’s t-test (P <0.001). hpf, hours post fertilization; WISH, whole-mount in situ hybridization.

Expression Data

Genes:	gcga ghrl ins sst2
Fish:	WT WT + MO1-ascl1b + MO3-neurod1
Knockdown Reagents:	MO1-ascl1b MO3-neurod1
Anatomical Terms:	pancreatic bud trunk embryonic structure
Stage Range:	14-19 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ascl1b + MO3-neurod1
Knockdown Reagents:	MO1-ascl1b MO3-neurod1
Observed In:	endocrine pancreas development
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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