FIGURE

Fig. 4

ID

ZDB-FIG-140523-45

Publication

Swift et al., 2014 - SoxF factors and Notch regulate nr2f2 gene expression during venous differentiation in zebrafish

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Fig. 4

Sox gene function is required for nr2f2 expression in the zebrafish vasculature. (A and B) Whole mount in situ hybridization of 24 hpf control (A) and sox7/sox18 double morpholino (DMO) injected (B) zebrafish embryo trunks probed for nr2f2. The inset numbers in panels B show the number of in situ-stained embryos exhibiting strongly reduced or absent vascular expression over the total number of embryos examined. (C) Quantitative RT-PCR measurement of nr2f2 and lyve1 gene expression in excised trunks (see Materials and Methods of 24 hpf control and sox7/sox18 DMO animals. (D) Quantitative RT-PCR measurement of nr2f2 gene expression in excised trunks (see Materials and Methods) of 24 hpf control MO injected, sox7/sox18 DMO injected, cloche mutant, and sox7/sox18 DMO injected cloche mutant animals. All quantitative RT-PCR values are shown normalized to control gene expression levels, which are set equal to 1. Rostral is to the left and dorsal is up in all image panels. Scale bars, 50µm.

Expression Data

Genes:	lyve1b nr2f2
Fish:	EKW npas4l^m378/m378 EKW + MO5-sox18 + MO5-sox7 npas4l^m378/m378 + MO5-sox18 + MO5-sox7
Knockdown Reagents:	MO5-sox7 MO5-sox18
Anatomical Terms:	posterior cardinal vein blood vessel endothelial cell spinal cord neural tube trunk
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO5-sox18 + MO5-sox7
Knockdown Reagents:	MO5-sox7 MO5-sox18
Observed In:	posterior cardinal vein venous blood vessel development
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 390, Swift, M.R., Pham, V.N., Castranova, D., Bell, K., Poole, R.J., Weinstein, B.M., SoxF factors and Notch regulate nr2f2 gene expression during venous differentiation in zebrafish, 116-25, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.