ZFIN ID: ZDB-FIG-140523-13
Van Ryswyk et al., 2014 - The role of inab in axon morphology of an identified zebrafish motoneuron. PLoS One   9(2):e88631 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 2

inab is dynamically expressed in PMNs and INs.

(A–K) Single confocal slices of embryos labeled with inab, islet, and neurotransmitter riboprobes (gads for GABA and vglut for glutamate). Diagonal lines represent somite boundaries. At 14 hpf, inab is coexpressed with neither islet1 (A) nor islet2a (E). Between 16 hpf and 24 hpf, inab is expressed in islet2a+ PMNs (circled cells in F–H) but not islet1+ PMNs (arrowheads in A–C). inab is expressed in the VeLD IN, as determined by coexpression with both GFP in mnx1:GFP transgenic embryos (arrowheads, I) and gad mRNA (arrowhead, J). At 24 hpf, inab is coexpressed with vglut (arrowheads, K) in a cell dorsal to the VeLD IN (circle, K). (L) Schematic of inab mRNA dynamics during early development. Between 16–24 hpf, inab is expressed in both CaP and VaP MNs and in VeLD INs. After 24 hpf, inab expression in CaP and VaP is downregulated, although it persists in VeLD and an additional, dorsally-located, glutamatergic IN. Scale bar, 5 μm in A–J; 10 μm in K.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP ml2Tg standard conditions 14-19 somites primary motor neuron IHC
14-19 somites VeLD IHC
inab WT standard conditions 10-13 somites neural tube ISH
14-19 somites CaP motoneuron ISH
14-19 somites Not Detected MiP motor neuron ISH
14-19 somites Not Detected RoP motor neuron ISH
14-19 somites VaP motor neuron ISH
14-19 somites VeLD ISH
Prim-5 CaP motoneuron ISH
Prim-5 glutamatergic neuron ISH
Prim-5 Not Detected MiP motor neuron ISH
Prim-5 Not Detected RoP motor neuron ISH
Prim-5 VaP motor neuron ISH
isl1a WT standard conditions 10-13 somites primary motor neuron ISH
14-19 somites MiP motor neuron ISH
14-19 somites RoP motor neuron ISH
Prim-5 MiP motor neuron ISH
Prim-5 RoP motor neuron ISH
isl2a WT standard conditions 10-13 somites primary motor neuron ISH
14-19 somites CaP motoneuron ISH
14-19 somites VaP motor neuron ISH
Prim-5 CaP motoneuron ISH
Prim-5 VaP motor neuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One