FIGURE

Fig. 5

ID

ZDB-FIG-140422-43

Publication

Holly et al., 2014 - Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development

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Fig. 5

sfrp1a/sfrp5 regulate retinal dorso-ventral patterning. To examine dorso-ventral retina patterning in sfrp1a/5 MO co-injected embryos, we employed in situ hybridization of ventral marker genes aldh1a3, ephb3a and vax2, as well as dorsal markers aldh1a2, bambia, and tbx5a at 28 and 48 hpf. At 28 hpf, sfrp1a/5 MO injected embryos display a normal domain of ventral markers compared to WT, though with slightly reduced levels (A–F). Analysis of 28 hpf dorsal retina patterning in sfrp1a/5 MO embryos also demonstrates a decreased expression of markers (G–L). By 48 hpf, in comparison to controls, sfrp1a/5 MO injected embryos display an expansion of both aldh1a3 and vax2 expression into the dorsal regions of the retina (M–P). Analysis at 48 hpf demonstrates a clear reduction of tbx5a and bambia expression domains and levels in sfrp1a/5 MO injected embryos (Q–T).

Expression Data

Genes:	aldh1a2 aldh1a3 bambia ephb3a tbx5a vax2
Fish:	WT WT + MO2-sfrp5 + MO3-sfrp1a
Knockdown Reagents:	MO2-sfrp5 MO3-sfrp1a
Anatomical Terms:	optic cup dorsal region optic cup ventral region retina retina dorsal region retina ventral region
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 388(2), Holly, V.L., Widen, S.A., Famulski, J.K., and Waskiewicz, A.J., Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development, 192-204, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.