Fig. 9

MiR-1 regulates EDN1 expression in HCC.

(A) The RNA expression of miR-1 in the edn1 transgenic fish and human specimens was determined by in-situ hybridization, and EDN1 protein expression in human specimen by IHC. The expression of miR-1 was at the high level in 3-month-old edn1 transgenic fish which is normal in pathological analysis (A1) and was decreased in the 9-month-old edn1 transgenic fish with HCC (A2). The liver tissue of the GFP-mCherry control zebrafish was stained with (A3) or without (A4) the U6 probe as the positive or negative control, respectively. The miR-1 signal was very intense in the normal human liver tissues (A5), but was weakened in the tissue of HCC at stage I to III tissues. (A6–8). The EDN1 signal was very weak in the normal liver tissue (A9), but became intense in the tissue of HCC at stage I to III (A10–12). The positive signal is shown in purple for miR-1 in-situ and brown for EDN1 IHC (200X). Scale bars: 50 μm. (B) Comparison of the expression of EDN1 and miR-1 in normal and HCC tissues. The staining intensity was scored from 0 to 4. _{** or ##}, P<0.001; _{*** or ###}, P<0.0001. (C) Correlation between EDN1 and miR-1 expression in the hepatic tissue of HCC patients.