Fig. 1
- ID
- ZDB-FIG-140417-3
- Publication
- Lu et al., 2014 - Overexpression of endothelin 1 triggers hepatocarcinogenesis in zebrafish and promotes cell proliferation and migration through the AKT pathway
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Generation and characterization of edn1 transgenic fish. (A) Diagram of the fabp10a:edn1-pA/CG2 construct that contains Tol2 sequences and the cmlc2:GFP expression cassette. (B) The Edn1 protein was expressed in the liver of the wild-type fish carrying the fabp10a:edn1-pA/CG2 transgene, as indicated by the expression of cmlc2:GFP in the heart (green fluorescence, 200X). Scale bars: 2 mm. (C) Quantitative RT-PCR analysis of edn1 mRNA expression in hepatocytes from 3-, 5-, 7-, 9- and 11-month-old edn1 transgenic fish (n = 3) and control fabp10a:GFP-mCherry transgenic fish. For qPCR analysis, a series of known PCR fragment (GFP) of known concentrations were used as the standard. The edn1 RNA concentration was calculated base on the standard curve. The differences between the edn1 transgenic fish and control fish were assessed using a two-tailed Student′s t-test. Asterisks *** represent the P value is less than 0.001. |
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| Stage: | Adult |