FIGURE

Fig. 3

ID

ZDB-FIG-140417-10

Publication

Wang et al., 2013 - Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish

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Fig. 3

Gli1 and Gli2a act redundantly to pattern the zebrafish myotome.

(A,E) Wild-type (WT); (B,F) gli2aⁱ²⁷⁶; (C,G) gli1^ts269 and (D,H) gli1^ts269; gli2aⁱ²⁷⁶ 24hpf embryos stained with anti Prox1a (green) and either mAb F59 (red: panels A–D) or 4D9 (red: panels E–H) to reveal slow-twitch muscle and MP fibres. (I–K) Transverse sections of embryos shown in panels E–G. (L–O) Dorsal views of the caudal regions of 18ss embryos hybridized with a probe for myod1 transcript. (L) WT; (M) gli2aⁱ²⁷⁶; (N) gli1^ts269 (O) gli1^ts269;gli2aⁱ²⁷⁶.

Expression Data

Gene:	myod1
Antibodies:	Ab-F59 Ab1-eng
Fish:	WT gli1^ts269/ts269 gli2a^i276/i276 gli1^ts269/ts269; gli2a^i276/i276
Anatomical Terms:	fast muscle cell muscle pioneer slow muscle cell somite
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gli1^ts269/ts269; gli2a^i276/i276
Observed In:	slow muscle cell somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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