FIGURE

Fig. 5

ID

ZDB-FIG-140416-18

Publication

Pantazis et al., 2010 - Second harmonic generating (SHG) nanoprobes for in vivo imaging

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Fig. 5

SHG nanoprobe targeting specificity. (A) Scheme of SHG nanoprobe conjugation to Cy5-coupled (green pentagon) antibody fragments via disulphide reduction and sulfhydryl-amine coupling. (B) Schematic representation of a transversal section of trunk of a 24 hpf zebrafish embryo showing Dystrophin protein localization to fiber ends (red). NT, neural tube; NC, notochord; V, vessels. (C–E) Immunostaining showing Dystrophin protein localization in a transversal tissue section using secondary antibody coupled to Cy5 (green) and BaTiO₃ (white). Both readouts—Cy5 immunofluorescence (C), and BaTiO₃ SHG signal (D)—label specifically Dystrophin. (E) Phalloidin labeling (red) is superimposed to show cell profiles in the transversal tissue section. Note that SHG immunostaining provides superior SNR of Dystrophin detection using a narrow emission filter. Bar = 30 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA