PUBLICATION

Second harmonic generating (SHG) nanoprobes for in vivo imaging

Authors
Pantazis, P., Maloney, J., Wu, D., and Fraser, S.E.
ID
ZDB-PUB-140226-1
Date
2010
Source
Proceedings of the National Academy of Sciences of the United States of America   107(33): 14535-14540 (Journal)
Registered Authors
Fraser, Scott E., Pantazis, Periklis (Laki)
Keywords
none
MeSH Terms
  • Animals
  • Barium Compounds/chemistry
  • Embryo, Nonmammalian/chemistry
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology
  • Immunohistochemistry
  • Microscopy, Atomic Force
  • Microscopy, Confocal/methods*
  • Microscopy, Electron, Scanning
  • Microscopy, Fluorescence, Multiphoton/methods*
  • Molecular Probes/chemistry*
  • Nanoparticles/chemistry
  • Nanoparticles/ultrastructure
  • Nanotechnology/methods*
  • Titanium/chemistry
  • Zebrafish/embryology
  • Zebrafish/metabolism
PubMed
20668245 Full text @ Proc. Natl. Acad. Sci. USA
Abstract

Fluorescence microscopy has profoundly changed cell and molecular biology studies by permitting tagged gene products to be followed as they function and interact. The ability of a fluorescent dye to absorb and emit light of different wavelengths allows it to generate startling contrast that, in the best cases, can permit single molecule detection and tracking. However, in many experimental settings, fluorescent probes fall short of their potential due to dye bleaching, dye signal saturation, and tissue autofluorescence. Here, we demonstrate that second harmonic generating (SHG) nanoprobes can be used for in vivo imaging, circumventing many of the limitations of classical fluorescence probes. Under intense illumination, such as at the focus of a laser-scanning microscope, these SHG nanocrystals convert two photons into one photon of half the wavelength; thus, when imaged by conventional two-photon microscopy, SHG nanoprobes appear to generate a signal with an inverse Stokes shift like a fluorescent dye, but with a narrower emission. Unlike commonly used fluorescent probes, SHG nanoprobes neither bleach nor blink, and the signal they generate does not saturate with increasing illumination intensity. The resulting contrast and detectability of SHG nanoprobes provide unique advantages for molecular imaging of living cells and tissues.

Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes