ZFIN ID: ZDB-PERS-090219-8
Pantazis, Periklis (Laki)
Email: p.pantazis@imperial.ac.uk
URL: http://www.pantazis-lab.org
Affiliation: Pantazis Lab
Address: Imperial College London Department of Bioengineering South Kensington Campus London SW7 2AZ
Country: United Kingdom
Phone: +44 (0)20 7594 6367
Fax:
ORCID ID: 0000-0002-8367-9332


BIOGRAPHY AND RESEARCH INTERESTS
Periklis (Laki) Pantazis obtained his Master’s degree in Biochemistry at the Leibniz University of Hanover/Germany. He then completed graduate studies in Biology and Bioengineering at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden/Germany, where he combined imaging with in silico
modelling to quantitatively analyse embryonic morphogenesis. As a Postdoctoral Scholar at the California Institute of Technology (Caltech) in Pasadena/USA, he
employed sophisticated imaging to address fundamental questions in Developmental Biology such as the generation of asymmetry in early embryo development.
He continued his academic career as an Assistant Professor of Biosystems Analysis at the Department of Biosystems Science and Engineering (D-BSSE) at ETH Zurich in Basel/Switzerland where he led the Laboratory of NanoBioImaging that developed a diverse imaging toolbox for the mechanistic understanding of development and disease and for the use in novel diagnostic and therapeutic strategies. His Lab fostered interdisciplinary projects in the fields of Developmental and Stem Cell Biology, Engineering, Chemistry and Optics.
In parallel, he started his teaching and mentoring career with lectures and workshops in “Interdisciplinary Biotechnology”, “Imaging in Systems Biology”, “Genetic Approaches in Biomedical Research”, etc. In summer of 2018, he transferred his Lab to the Department of Bioengineering and joined Imperial College London as a Senior Lecturer (Associate Professor).


PUBLICATIONS
Sonay, A.Y., Kalyviotis, K., Yaganoglu, S., Unsal, A., Konantz, M., Teulon, C., Lieberwirth, I., Sieber, S., Jiang, S., Behzadi, S., Crespy, D., Landfester, K., Roke, S., Lengerke, C., Pantazis, P. (2021) Biodegradable Harmonophores for Targeted High-Resolution In Vivo Tumor Imaging. ACS nano. 15(3):4144-4154
Malkinson, G., Mahou, P., Chaudan, É., Gacoin, T., Sonay, A.Y., Pantazis, P., Beaurepaire, E., Supatto, W. (2020) Fast In Vivo Imaging of SHG Nanoprobes with Multiphoton Light-Sheet Microscopy. ACS photonics. 7:1036-1049
Mohr, M.A., Argast, P., Pantazis, P. (2016) Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins. Nature Protocols. 11:2419-2431
Mohr, M.A., Pantazis, P. (2016) Single neuron morphology in vivo with confined primed conversion. Methods in cell biology. 133:125-138
Dempsey, W.P., Hodas, N.O., Ponti, A., Pantazis, P. (2015) Determination of the source of SHG verniers in zebrafish skeletal muscle. Scientific Reports. 5:18119
Dempsey, W.P., Georgieva, L., Helbling, P.M., Sonay, A.Y., Truong, T.V., Haffner, M., Pantazis, P. (2015) In vivo single-cell labeling by confined primed conversion. Nature Methods. 12(7):645-8
Pantazis, P., Supatto, W. (2014) Advances in whole-embryo imaging: a quantitative transition is underway. Nature reviews. Molecular cell biology. 15:327-39
Dempsey, W.P., Qin, H., Pantazis, P. (2014) In Vivo Cell Tracking Using PhOTO Zebrafish. Methods in molecular biology (Clifton, N.J.). 1148:217-28
Mikut, R., Dickmeis, T., Driever, W., Geurts, P., Hamprecht, F.A., Kausler, B.X., Ledesma-Carbayo, M.J., Marée, R., Mikula, K., Pantazis, P., Ronneberger, O., Santos, A., Stotzka, R., Strähle, U., and Peyriéras, N. (2013) Automated Processing of Zebrafish Imaging Data: A Survey. Zebrafish. 10(3):401-21
Culi-Viskota, J., Dempsey, W.P., Fraser, S.E., and Pantazis, P. (2012) Surface functionalization of barium titanate SHG nanoprobes for in vivo imaging in zebrafish. Nature Protocols. 7(9):1618-1633
Dempsey, W.P., Fraser, S.E., and Pantazis, P. (2012) PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration. PLoS One. 7(3):e32888
Caneparo, L., Pantazis, P., Dempsey, W., and Fraser, S.E. (2011) Intercellular bridges in vertebrate gastrulation. PLoS One. 6(5):e20230
Pantazis, P., Maloney, J., Wu, D., and Fraser, S.E. (2010) Second harmonic generating (SHG) nanoprobes for in vivo imaging. Proceedings of the National Academy of Sciences of the United States of America. 107(33):14535-14540

NON-ZEBRAFISH PUBLICATIONS
*contributed equally
#corresponding author

Refereed Primary Papers
In Revision
[49] Yaganoglu, S., Helassa, N., Gaub, B.M., Welling, M.A., Shi, J., Müller, D.J., Török, K., and Pantazis, P.#, “GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution”.
Invention filed: E.P. patent pending (2016, EP 2016/191538).
[48] Sonay, A.Y., Yaganoglu, S., Konantz, M., Teulon, C., Jiang, S., Behzadi, S., Crespy, D., Landfester, K., Roke, S., Lengerke, C., and Pantazis, P.#, “Biodegradable harmonophores for targeted high-resolution in vivo tumor imaging”.
Invention filed: E.P. patent pending (2016, EP 2016/191538).
Nominated for the SPARK Award 2017
In Press
[47] Welling, M.A.*, Mohr, M.A.*, Ponti, A., Sabater, L.R., Boni, A., Kitazawa, Y., Liberali, P., Peters, A., Pelczar, P., and Pantazis, P.# (2019), “High fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins”. eLIFE
2018
[46] Slenders, E., Bové, H., Urbain, M., Mugnier, Y., Sonay. A.Y., Pantazis, P., Bonacina, L., Vanden Berghe, P., vandeVen, M., and Ameloot, M.#, (2018) ”Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells.” J Phys Chem Lett. 20:6112-6118.
[45] Sugiyama, N.*, Sonay, A.Y.*, Tussiwand, R., Cohen, B., and Pantazis, P.#, (2018) “Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging”. Small 8:1703386.
2017
[44] Nugraha, B., Mohr, M.A., Ponti, A., Emmert, M.Y., Weibel, F., Hoerstrup, S.P., Moll, S., Certa, U., Prunotto, M., and Pantazis, P.#, (2017) “Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture”. Scientific Reports 7:14490.
[43] Mohr, M.A., Kobitski, A.Yu., Sabater, L.R., Nienhaus, K., Obara, C.J, Lippincott-Schwartz, J., Nienhaus, G.U.#, and Pantazis, P.#, (2017) “Rational Engineering of Photoconvertible Fluorescent Proteins for Dual-Color Fluorescence Nanoscopy Enabled by a Triplet-State Mechanism of Primed Conversion”. Angew. Chem. Int. Ed. 56:11628–11633.
Featured in: Bergman, F. (2017) “Red fluorescence in two steps” ETH Zurich News.
Invention filed: U.S. provisional patent submitted (2017, Application Serial No. 62/446,023).
[42] Zhang,W., Lohman, A.W., Zhuravlova, Y., Lu, X., Wiens, M.D., Hoi, H., Yaganoglu, S., Mohr, M.A., Kitova, E.N., Klassen, J.S., Pantazis, P., Thompson, J.R., and Campbell, R.E.#, (2017) “Optogenetic control with a photocleavable protein” Nature Methods 14:391–394.
2016
[41] Mohr, M.A., Argast, P., and Pantazis, P.#, (2016) “Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins” Nature Protocols 11:2419-2431.
Invention W.O. patent pending (2014, WO 2014/147211) and E.P. patent pending (2014, EP 2014/055669) licensed to: AHF Analysentechnik, Tübingen/Germany. Press release.
2015
[40] Dempsey, W.P.*, Hodas, N.O.*, Ponti, A., and Pantazis, P.#, (2015) “Determination of the source of SHG verniers in zebrafish skeletal muscle” Scientific Reports 5:18119.
[39] Dempsey, W.P.*, Georgieva, L.*, Helbling, P.M., Sonay, A.Y., Truong, T.V., Haffner, M., and Pantazis, P.#, (2015) “In vivo single cell labeling by confined primed conversion” Nature Methods 12:645–648.
Featured in 11 news outlets and scientific blogs among them: Bergman, F. (2015) “Chameleon proteins make individual cells visible” ETH Zurich News.
Invention W.O. patent pending (2014, WO 2014/147211) and E.P. patent pending (2014, EP 2014/055669) licensed to: DNAWrite, Cambridge/UK and Viventis Microscopy, Lausanne/CH.
2012
[38] Culic-Viskota, J.*, Dempsey, W.P.*, Fraser, S.E., and Pantazis, P.#, (2012) “Surface functionalization of barium titanate SHG nanoprobes for in vivo imaging in zebrafish” Nature Protocols 7:1618-33.
[37] Dempsey, W.P., Fraser, S.E., and Pantazis, P.#, (2012) “PhOTO Zebrafish: A transgenic resource for in vivo lineage tracing during development and regeneration” PLoS ONE 7:e32888.
Featured in: "PhOTO zebrafish: a transgenic resource for in vivo lineage tracing during development and regeneration, Significance statement" Global Medical Discovery August 25 2012
2011
[36] Plachta, N., Bollenbach, T., Pease, S., Fraser, S.E., and Pantazis, P.#, (2011) “Oct4 kinetics predict cell lineage patterning in the early mammalian embryo” Nature Cell Biology 13:117-23.
Provided front cover of the journal and featured in: Zernicka-Goetz, M., (2011) "Proclaiming fate in the early mouse embryo" Nature Cell Biology 13:112-4.
[35] Caneparo, L.*, Pantazis, P.*, Dempsey, W.P., and Fraser, S.E., (2011) “Intercellular bridges in vertebrate gastrulation” PLoS ONE 6:e20230.
2010
[34] Pantazis, P.#, Maloney, J., Wu, D., and Fraser, S.E., (2010) “Second Harmonic Generating (SHG) nanoprobes for in vivo imaging” Proc Natl Acad Sci USA 107:14535-40.
Featured in: Cohen, B., (2010) “Biological imaging: Beyond fluorescence” Nature 467:407-8; Evanko, D., (2010) “Microscope harmonies” Nature Methods 7:779.
[33] Tu, C., Ma, X., Pantazis, P., Kauzlarich, S.#, and Louie, A.#, (2010) “Paramagnetic, silicon quantum dots for magnetic resonance and two photon imaging of macrophages” J Am Chem Soc 132:2016-23.
2008
[32] Bollenbach, T.*, Pantazis, P.*, Kicheva, A.*, Boekel, C., Kruse, K., Gonzalez-Gaitan, M.#, and Juelicher, F.#, (2008) “Precision of the Dpp gradient” Development 135:1137-46.
2007
[31] Kicheva, A.*, Pantazis, P.*, Bollenbach, T.*, Kalaidzidis, Y., Bittig, T., Juelicher, F.#, and Gonzalez-Gaitan, M.#, (2007) “Kinetics of morphogen gradient formation” Science 315(5811):521-5.
Featured in: Kritikou, E., (2007) “Reaching one’s range” Nature Reviews Molecular Cell Biology 8:181.
[30] Pantazis, P.#, and González-Gaitán, M., (2007) “Localized multiphoton photoactivation of paGFP in Drosophila wing imaginal discs” J Biomed Opt 12:044004.
[29] Bollenbach, T., Kruse, K., Pantazis, P., Gonzalez-Gaitan, M., Juelicher, F.#, (2007) “Morphogen transport in epithelia.” Phys Rev E Stat Nonlin Soft Matter Phys.75 (1 Pt 1):011901.
2005
[28] Bollenbach, T., Kruse, K., Pantazis, P., Gonzalez-Gaitan, M., and Juelicher, F.#, (2005) “Robust formation of morphogen gradients” Phys Rev Lett 94:018103(1-4).
[27] Kruse, K.*, Pantazis, P.*, Bollenbach, T., Juelicher, F.#, and Gonzalez-Gaitan, M.#, (2004) “Dpp gradient formation by Dynamin-dependent endocytosis: receptor trafficking and the diffusion model” Development 131:4843-56.
1999
[26] Tsikas, D.#, Sandmann, J., Holzberg, D., Pantazis, P., Raida, M., and Frolich, J.C., (1999) “Determination of S-nitrosoglutathione in human and rat plasma by high-performance liquid chromatography with fluorescence and ultraviolet absorbance detection after precolumn derivatization with o-phthalaldehyde” Anal Biochem 273:32-40.

Refereed Reviews Publications
2018
[25] Mohr, M.A., and Pantazis, P.#, (2018) “Primed conversion: the new kid on the block for
photoconversion” Chemistry 33:8268-8274.
2016
[24] Welling, M., Ponti, A., and Pantazis, P.#, (2016) “Symmetry breaking in the early mammalian embryo: the case for quantitative single-cell imaging analysis” MHR-Molecular 
Human Reproduction 22:172-181.
2014
[23] Pantazis, P.#, and Supatto W., (2014) “Advances in whole-embryo imaging: a quantitative transition is underway” Nature Reviews Molecular Cell Biology 15:327–39.
2013
[22] Mikut R.#, Dickmeis T., Driever W., Geurts P., Hamprecht F.A., Kausler B.X., Ledesma-Carbayo M.J., Marée R., Mikula K., Pantazis P., Ronneberger O., Santos A., Stotzka R., Strähle U., and Peyriéras N., (2013) “Automated processing of zebrafish imaging data: A survey” Zebrafish 10:401-21.
2012
[21] Pantazis, P.#, and Bollenbach, T., (2012) “Transcription factor kinetics and the emerging asymmetry in the early mammalian embryo” Cell Cycle 11:2055-8.
[20] Dempsey, W.P., Fraser, S.E., and Pantazis, P.#, (2012) “SHG nanoprobes: Advancing harmonic imaging in biology” Bioessays 34:351-60.
2004
[19] Dudu, V.*, Pantazis, P.*, and Gonzalez-Gaitan, M.#, (2004) “Membrane traffic during embryonic development: epithelial formation, cell fate decisions and differentiation” Curr Opin Cell Biol 16:407-414.

Refereed Conference Publications
2018
[18] Nienhaus, K., Mohr, M.A., Kobitski, A, Y., Sabater, L.R., Obara, C.J., Lippincott-Schwartz, J., Nienhaus, G.U.#, and Pantazis, P.# (2018) "Primed Green-to-Red Photoconversion of Fluorescent Proteins Occurs via a Triplet State." Biophysical Journal 114:533a.
2017
[17] Sonay, A.Y., and Pantazis, P.#, (2017) “Bioinspired Second Harmonic Generation” Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics, 104110D.
2009
[16] Pantazis, P.#, Pu, Y., Psaltis, D., and Fraser, S.E., (2009) “Second Harmonic Generating (SHG) Nanoprobes: A new tool for biomedical imaging” Proc. SPIE 7183:71831-5.

Refereed Books and/or Book Chapters
2016
[15] Mohr, M.A., and Pantazis, P.#, (2016) “Single neuron morphology in vivo with confined primed conversion” Methods Cell Biology 133:125-38.
Figure selected as cover image for: Volume 1, Methods in Cell Biology, The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology 4th Edition.
2014
[14] Dempsey, W.P., Qin, H., and Pantazis, P.#, (2014) “In vivo cell tracking using PhOTO Zebrafish” Methods Molecular Biology 1148:217-28.

Patents
2018
[13] Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L. “Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” U.P. patent granted (15 May 2018, US 90,971,136).
[12] Pantazis, P., Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” U.S. patent granted (20 Feb 2018, US 9,971,136).
2017
[11] Pantazis, P. and Yaganoglu, S., “Piezo1-based fluorescent reporter” E.P. patent pending (2017, EP 2017/17210479.6).
[10] Mohr, M.A., and Pantazis, P., “Engineered photoconvertible fluorescent proteins (pcFPs) for primed conversion” U.S. patent pending (2017, US 62/446,023).
2016
[9] Pantazis, P., Sonay, A.Y., Landfester, K., and Crespy, D., “Biodegradable second harmonic generating nanoprobes” E.P. patent pending (2016, EP 2016/191538).
Among the top 20 innovations of ETH Zurich in 2016
[8] Pantazis, P., Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” U.S. patent granted (25 Oct 2016, US 9,476,830).
[7] Pantazis, P., Culic-Viskota, J., Dempsey, W.P., and Fraser, S.E. “Functionalization of and use of functionalized second harmonic generating nanoprobes” U.S. patent granted (31 May 2016, US 9,352,055).
2015
[6] Pantazis, P., Culic-Viskota, J., Dempsey, W.P., and Fraser, S.E. “Functionalization of and use of functionalized second harmonic generating nanoprobes” U.S. patent granted (29 Dec 2015, US 9,221,919).
[5] Pantazis, P., Masmanidis, S., and Fraser, S.E. “Multipurpose Analysis Using Second Harmonic Generating Nanoprobes” U.S. patent granted (03 Feb 2015, US 8,945,471 B2).
2014
[4] Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L. “Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” W.O. patent pending (2014, WO 2014/147211).
[3] Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L. “Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” E.P. patent pending (2014, EP 2014/055669).
2010
[2] Pantazis, P., Masmanidis, S., and Fraser, S.E. “Multipurpose Analysis Using Second Harmonic Generating Nanoprobes” W.O. patent pending (2010, WO 2010/090844).
2008
[1] Pantazis, P., Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” W.O. patent pending (2008, WO 2008/140584).