ZFIN ID: ZDB-FIG-140404-7
Gray et al., 2014 - Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations. Developmental Biology   386(1):72-85 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 7-13 to Days 14-20

Fig. 5 Segmentation of the vertebral column and localization of osteoblasts proceeds on a dysmorphic notochord template. Alcian blue stained larvae (14dpf) (A–B′′′) with lateral insets (red dashed box; A′, B′) and ventral insets (green dashed box; A′′′, B′′′). (A–A′′′) WT larvae display well-formed and segmented centra. (B–B′′′) levvu41-/- larvae display abberant apposition of centra (red brackets) and hemicentra (red arrow). Confocal projections of zebrafish vertebral column at 21dpf, illustrating boney matrix (alizarin red in magenta) and osteoblasts (Tg(sp7:EGFP)b1212 in green) (C–F′). (C, D) WT vertebrae and associated osteoblasts are well segmented along the vertebral axis. (E, E′) An individual levvu41-/- mutant displaying segmented colocalization of osteoblast and centra, including malformed hemivertebrae (yellow arrows) that continues to have mostly well segmented osteoblasts (E′), excepting some ectopic osteoblast-mineral formations in regions previously devoid of osteoblasts or mineral (E′, yellow asterix). (F and F′) An individual levvu41-/- mutant displaying regions of severe notochord bending with aberrantly apposed osteoblasts (F, yellow bracket), those progresses to a region with more complete osteoblast coverage (F′, yellow bracket). (C,D) 10dpf; ~SL3-4 (E,F) 10dpf; ~SL3-4 (E′, F′) 14dpf; ~SL4-5. Scale bars for (A, B, A3, B3)=1 mM; (C–F′) and (A′, A′′′,B′,B′′′)=100 mM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP b1212Tg standard conditions Days 7-13 osteoblast IFL
col8a1avu41/vu41; b1212Tg standard conditions Days 7-13 osteoblast IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
col8a1avu41/vu41 standard conditions Days 14-20 centrum disorganized, abnormal
Days 14-20 centrum irregular spatial pattern, abnormal
Days 14-20 centrum malformed, abnormal
col8a1avu41/vu41; b1212Tg standard conditions Days 7-13 vertebra malformed, abnormal
Days 7-13 vertebral column deviation, abnormal
Days 7-13 vertebral column osteoblast mislocalised, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 386(1), Gray, R.S., Wilm, T.P., Smith, J., Bagnat, M., Dale, R.M., Topczewski, J., Johnson, S.L., and Solnica-Krezel, L., Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations, 72-85, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.