ZFIN ID: ZDB-FIG-140404-5
Gray et al., 2014 - Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations. Developmental Biology   386(1):72-85 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Days 45-89

Fig. 3 Homozyogus col8a1a mutant adults display vertebral fusions and scoliosis. MicroCT imaging of WT (A–B′) and col8a1a mutant zebrafish (C–D′) adults (45dpf) (A–E′′) in both lateral views (A, C, E–E′′) with insets (A′ and C′) and dorsal views (B and D) with insets (B′, D′) (A, A′, and B′) WT vertebrae with one neural and hemal arches per vertebral body. (B) Representative scoliosis in the tail of a WT adult (yellow arrowhead). (C and C′) Representative levvu41-/- zebrafish with VM (red arrowheads). (D and D′) Representative levvu41-/- multiple scoliosis along the axis (yellow arrowheads) and (E–E′′) Representative individuals scored for the presence of individual VM (red arrowheads) or complete fusion of vertebral column (red bracket) in the thoracic or caudal regions (as number of fused regions). (F) Graphed categorical data of VM from all lev alleles and unaffected WT siblings in thoracic (T) and caudal (C) regions. All scale bars (A–D), (A′–D′), and (E–E′′)=1 mM.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
col8a1am531/m531 standard conditions Days 45-89 vertebra fused with vertebra, abnormal
col8a1avu105/vu105 standard conditions Days 45-89 vertebra fused with vertebra, abnormal
col8a1avu41/vu41 standard conditions Days 45-89 vertebra fused with vertebra, abnormal
Days 45-89 vertebral column deviation, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 386(1), Gray, R.S., Wilm, T.P., Smith, J., Bagnat, M., Dale, R.M., Topczewski, J., Johnson, S.L., and Solnica-Krezel, L., Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations, 72-85, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.