FIGURE

Fig. S1

ID

ZDB-FIG-131210-29

Publication

Jones et al., 2013 - Modelling human Wiskott-Aldrich syndrome protein mutants in zebrafish larvae using live in vivo imaging

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Fig. S1

WASp1 is expressed in the hematopoietic lineages of Zebrafish larvae with WASp mutants showing no developmental defect in leukocyte numbers or distribution. (A) Whole-mount in situ hybridization for WASp1 in 1 dpf larvae, indicating expression in the early hematopoietic lineage (white arrowheads). (B) WASp1 antibody immunostaining depicting co-localisation with the transgenic neutrophil marker, lyz:DsRED in 3 dpf larvae. (C) WASp1 mutant has a normal distribution of leukocytes, as shown by L-Plastin immunostaining (red) in an mpx:GFP neutrophil transgenic line (green). (D) WASp1 mutant has a normal level of expression of the leukocyte marker Lplastin at 3 dpf, as shown by qPCR, indicating normal numbers of leukocytes. (E) RT-PCR showing transgenic expression of hWASp in “rescued” larvae is comparable to native levels of WASp1 transcript in WT larvae, with corresponding EFα control. Error bars (s.d). Scale bars: A, 100 μm; B, 10 μm; C, 200 μm.

Expression Data

Genes:	DsRed2 GFP wasb
Antibodies:	Ab1-wasb Ab2-lcp1
Fish:	WT i114Tg nz50Tg wasb^{hu3280/hu3280}; i114Tg
Anatomical Terms:	hematopoietic system leukocyte neutrophil
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.