FIGURE

Fig. S7

ID

ZDB-FIG-131031-21

Publication

Jao et al., 2013 - Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

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Fig. S7

Biallelic disruption of golden (gol) by Cas9 targeting a second location at the gol locus (gol target 2) generates similar mosaic pigmentation phenotypes. gol gRNA (gol target 2) (50 pg) and nls-zCas9-nls RNA (150 pg) were injected into wild-type embryos. Lateral (A–C) or dorsal (A2–C2) views of wild-type (A and A2) and gol-targeted embryos (B–C2) at 2 dpf. Whereas the wild-type embryo showed darkly pigmented retinal pigment epithelium (RPE), gol-targeted embryos had patches of unpigmented cells in RPE. The skin melanophores in gol-targeted embryos were also hypopigmented. The phenotypes were indistinguishable from those generated by the other gol-targeted gRNA shown in Fig. 4. (Scale bars: in C, 300 μm; in C2, 500 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA