FIGURE

Fig. 4

ID
ZDB-FIG-130802-34
Publication
Collin et al., 2013 - ZNF408 is mutated in familial exudative vitreoretinopathy and is crucial for the development of zebrafish retinal vasculature
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Fig. 4

Cotransfection analysis of WT and p.His455Tyr mutant ZNF408. (A–C) Immunocytochemical analysis of transiently transfected HA-tagged WT ZNF408 proteins. Representative examples show WT ZNF408 expression inside the cell nuclei (arrows). Images are presented as DAPI (staining cell nuclei) (A), anti-HA (detecting HA-tagged ZNF408 proteins) (B), and merged pictures (DAPI in blue, HA-tagged ZNF408 in red) (C). (D–F) Fluorescence analysis of transiently transfected eCFP-p.His455Tyr mutant ZNF408 proteins. Mutant ZNF408 is located mainly outside the cell nuclei (indicated by arrows). Images are presented as DAPI (staining cell nuclei) (D), eCFP (detecting eCFP-ZNF408 mutant proteins) (E), and merged pictures (DAPI in blue, eCFP-tagged p.His455Tyr ZNF408 in green) (F). (G–J) Combined analysis of COS-1 cells cotransfected with HA-tagged WT ZNF408 as well as eCFP-p.His455Tyr mutant ZNF408 proteins. In cells that express both the mutant and the WT ZNF408, the WT ZNF408 is also retained in the cytoplasm (I and J). Images are presented as DAPI (staining cell nuclei) (G), eCFP (detecting eCFP-p.His455Tyr ZNF408 mutant proteins (H), anti-HA (detecting HA-tagged WT ZNF408 proteins) (I), and merged pictures (DAPI in blue, HA-tagged WT ZNF408 in red, eCFP-p.His455Tyr ZNF408 in green) (J).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA
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